Pengaruh variasi konsentrasi larutan belimbing wuluh (Averrhoa bilimbi) terhadap uji aktivitas antimikroba secara In vitro

Desna Ayu Wijayanti; Osfar Sjofjan; Irfan H. Djunaidi

doi:10.21776/ub.jiip.2019.029.01.02

Pengaruh variasi konsentrasi larutan belimbing wuluh (Averrhoa bilimbi) terhadap uji aktivitas antimikroba secara In vitro

Desna Ayu Wijayanti, Osfar Sjofjan, Irfan H. Djunaidi

Abstract

The purpose of this research to determine the pH value and the antimicrobial activity of bilimbi extract (LBW) as a substitute for antibiotics. The method was field experiment using Completely Randomize Design with 4 treatments and 6 replications. The treatments were consist of P0(+) = antibiotic, P1= LBW 10%, P2= LBW 20% and P3= LBW 30%. Bilimbi extract used from bilimbi powder which is then dissolved. The measured variables were pH value and inhibition zone diameter of Escherichia coli, Salmonella sp. and Lactic Acid Bacteria. The data were analyzed by ANOVA and continued by Duncan’s Multiple Range Test (DMRT). The result of this research showed that LBW with higher concentration produces low pH value and the treatments between variation of concentration gave significantly different effect (P<0.01) on diameter of inhibition zone for Escherichia coli, Salmonella sp. and Lactic Acid Bacteria. The conclusion of this research was 30% concentration of LBW produces the lowest pH value, so that it can be inhibit the bacterial growth and has the potential as a substitute for antibiotics based on the result of antimicrobial activity that produces the best activity.

Keywords

antimicrobial activity; bilimbi; inhibition zone diameter; pH

Full Text:

PDF

References

Andriani., Darmono., dan Kurniawati, W. 2007. Pengaruh asam asetat dan asam laktat sebagai antibakteri terhadap bakteri Salmonella sp. yang diisolasi dari karkas ayam. Seminar Nasional Teknologi Peternakan dan Veteriner, (pp. 930-934). Bogor.

Chaveerach, P., Keuzenkamp, D.A., Urlings, H.A., Lipman, L.J., and Knapen, F. 2002. In vitro study on the effect of organic acids on Cam-pylobacter jejuni/coli populations in mixtures of water and feed. Poultry Science, 81(5), 621-628. doi: https://doi.org/10.1093/ps/81.5.621

Datu, J. T., Mita, N., dan Rusli, R. 2015. Aktivitas antibakteri sari buah belimbing wuluh (Averrhoa bilimbi linn.) terhadap bakteri Pseudomonas aeruginosa dan Staphylococcus epi-dermis. Prosiding Seminar Nasional Kefarmasian Ke-1, (pp. 1-9). Univer-sitas Mulawarman, Kalimantan Ti-mur.

Dibner, J.J., and Buttin, J. 2002. Use of organic acids as a model to study the impact of gut microflora on nutrition and metabolism. The Journal of Ap-plied Poultry Research. 11(4), 453-463.doi:https://doi.org/10.1093/japr/11.4.453

Frazier, W.C., and Westhoff, D.C. 1998. Food Microbiology (4th ed.). Mc Graw-Hill Book Co, New York.

Kurniawan, D. 2018. Aktivitas antimikroba dan antioksidan ekstrak tepung daun dan buah mengkudu (Morinda citrifo-lia). Jurnal Ilmu-Ilmu Peternakan, 28(2), 105-111.

doi:10.21776/ub.jiip.2018.028.02.02.

Luckstadt, C., and Mellor, S. 2011. The use of organic acids in animal nutrition, with special focus on dietary pottasi-um diformate under European and Austral-Asian conditions. Recent Ad-vances in Animal Nutrition. 18(11), 123-130.

Mohammed, H. A. 2016. Effect of utiliza-tion organic acid supplement on broiler (ros-308) feeding at pre-starter and starter period breeding on basic performance parameters. Internation-al Journal of Advanced Research in Biological Sciences, 3(6), 76-81. doi: s-o-i.org/1.15/ijarbs-2016-3-6-10

Rahmawati, N., Sudjarwo, E., dan Wido-do, E. 2014. Uji aktivitas antibakteri ekstrak herbal terhadap bakteri Esch-erichia coli. Jurnal Ilmu-Ilmu Peter-nakan, 24(3), 24-31.

Rahmi, F. 2015. Pembuatan bubuk asam sunti melalui optimasi suhu dan lama pengeringan. Jurnal Teknologi dan Industri Pertanian Indonesia, 7(2), 53-56. doi:dx.doi.org/10.17969/jtipi.v7i2.3278.

Soleha, T. A. 2015. Uji kepekaan terhadap antibiotik. Jurnal Kedokteran, 5(9), 119-123.

Sutrisna, R., Ekowati, C.N., dan Sinaga, E. 2015. Pengaruh pH terhadap produksi antibakteri oleh bakteri asam laktat dari usus itik. Jurnal Penelitian Pertanian Terpadu, 15(3), 234-238.

doi: 10.25181/jppt.v15i3.135.

Yuk, H.G., and Marshall, D.L. 2005. Influ-ence of acetic, citric, and lactic acids on Escherichia coli O157:H7 mem-brane lipid composition, verotoxin secretion, and acid resisteance in simulated gastric fluid. Journal of Food Protection, 68(4), 673-679. doi: 10.4315/0362-028X-68.4.673.

Yuliansyah, M. F., Widodo, E., dan Djunaidi, I. H. 2014. Pengaruh penambahan sari buah belimbing wuluh (averrhoa bilimbi l) sebagai acidifier dalam pakan terhadap kuali-tas internal telur ayam petelur. Jurnal Nutrisi Ternak. 1(1), 19-26.

DOI: http://dx.doi.org/10.21776/ub.jiip.2019.029.01.02