Pengaruh berbagai formulasi pengencer dasar air kelapa terhadap kualitas semen cair sapi PO (Peranakan Ongole) selama simpan dingin
DOI:
https://doi.org/10.21776//ub.jiip.2019.029.01.01Keywords:
acrosom reaction, capacitation, coconut water, motility.Abstract
Coconut water extender is the solution to the difficulty and high cots of procurement of diluents. The objective of this study was to examine the effect of various formulations of coconut water extender on quality of PO bull sperm during storage in 4-5 oC. This study was carried out at Animal Reproduction Laboratory of Faculty of Animal Husbandry Brawijaya University and Laboratory of Beef Cattle Research Center Grati Pasuruan on March to April, 2018. The study method used laboratory experimental. The experimental design was Factorial Randomized Completely Block Design and the data were analyzed by Analyze of Variance. There are four treatments in this study (P0 = CEP-3 + 20 % of yolk, P1 = Coconut Water + 20% of yolk, P2 = Coconut Water + 20% of yolk + 0,4% white egg + fructose 1 mg/ml, dan P3 = Coconut Water + 20% of yolk + 0,4% white egg + fructose 2 mg/ml) and ten replications of each treatment. There were significant differences in sperm motility between CEP-3 extender and coconut water-based with various formula extenders during chilled storage at 2-5 oC. But the treatment between coconut water-based formulas did not show a significant difference. Coconut water extenders are able to maintain sperm motility up to day 5 with values above 57%.References
Adnani, Luh P. D. H, W. Bebas, M. K. Budiasa. 2012. Penambahan bovine serum albumin pada pengencer kuning telur terhadap motilitas dan daya hidup spermatozoa anjing. In-donesia Medicus Veterinus, 1(4), 519–529.
Audia, R. P., M. A. Salim, N. Isnaini dan T. Susilawati. 2017. Pengaruh perbe-daan kematangan air kelapa hijau se-bagai bahan pengencer yang dit-ambah 10% kuning telur terhadap kualitas semen cair kambing Boer. Jurnal Ternak Tropika, 18(1), 58-68.
Ax, R., M. Dally, B. Didion, R. Lenz, C. Love, D. Varner, Hafez, and M. Bel-lin. 2008. Semen Evaluation. In Re-production in Farm Animal (7th edi-tion). Edited by Hafez, E.S.E. Co. Director. Reproductive Health Kiawah Island. South Carolina. USA: 365-370.
Aziz, A. F., M. A. Salim, N. Isnaini, A.P. A. Yekti, dan T. Susilawati. 2018. Pengaruh pengencer air kelapa tua yang berbeda varietas terhadap kuali-tas semen cair kambing Boer pada penyimpanan 3-5 oC. Jurnal Ilmu-Ilmu Peternakan, 28(2), 112 – 120.
Ducha, N., T. Susilawati, Aulanni’am, dan S. Wahjuningsih. 2013. Motilitas dan viabilitas spermatozoa sapi Limousin selama penyimpanan pada refrigera-tor dalam pengencer CEP-2 dengan suplementasi kuning telur. Jurnal Kedokteran Hewan, 7(1), 5-8.
Garner, D.L. and E.S.E. Hafez. 2008. Spermatozoa and Seminal Plasma. In Reproduction in Farm Animals (7th edition). Edited by E.S.E Hafez and B. Hafez. 2008. Lippincott & Wil-liams. Baltimore, Marryland. USA: 96-109.
Hernandez, P.J.E., Fernandez R.F., Rodri-guez S.J.L, Soto M.Y.G., Verona J.E.H., Garcia R.A.D. 2012. Post-thaw acrosomal viability and reaction in sperm obtained from equine epidi-dymis tail. Rev Salud Anim, 34(2), 84-88.
Ismaya. 2014. Bioteknologi Inseminasi Bu-atan pada Sapi dan Kerbau. Gadjah Mada University Press. Yogyakarta. ISBN 979-420-848-5.
Moore AI, Squires EL, Graham JK. 2005. Adding cholesterol to the stallion sperm plasma membrane improve cryosurvival. Cryobiology, 51(3), 241-249.
Morado, S., Pereyra, V., Breininger, E., Sara, R. and Cetica, P. 2015. Study of sperm evaluation parameters to es-timate cryopreserved bovine semen fertility. Austin Journal of Veterinary Science & Animal Husbandry, 2(1), 1005.
Mugiyati, Salin M. A., N. Isnaini, dan T. Susilawati. 2017. Pengaruh air kelapa merah yang muda dan tua sebagai pengencer terhadap kualitas semen kambing Boer selama penyimpanan dingin. Jurnal Ternak Tropika, 18(1), 20-26.
Muhammad, D., T. Susilawati, S. Wahjun-ingsih. 2016. Pengaruh penggunaan CEP-2 dengan suplementasi kuning telur terhadap kualitas spermatozoa sapi FH (Frisian Holstein) kualitas rendah selama penyimpanan suhu 4-5oC. Jurnal Ternak Tropika, 17(1), 66-76.
Susilawati, T. 2011. Spermatology. Uni-versitas Brawijaya (UB) Press. Ma-lang. ISBN 978-602-8960-04-5.
Susilawati, T. 2013. Pedoman Inseminasi Buatan pada Ternak. Universitas Brawijaya (UB) Press. Malang. ISBN 978-602-203-458-2.
Susilawati, T., D. Ratnawati, N. Isnaini, Kuswati and A. P. A. Yekti. 2018. Character of liquid semen motility in various diluents on Balinese cattle during cold storage. Asian Journal of Microbiology, Biotechnology & Envi-ronmental Sciences, 20(1), 166-172.
Vigliar R., V. L. Sdepanian, U. Fagundes-Neto. 2006. Biochemical profile of coconut water from coconut palms planted in an Inland Region. Jornal de Pediatria, 82(4), 308-312.
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