Stearoyl-CoA Desaturase (SCD) Gene Diversity and Its Association with Fatty Acids in Bali Cattle Using PCR-RFLP Method
DOI:
https://doi.org/10.21776/ub.jiip.2025.035.01.1Keywords:
Bali cattle, fatty acid composition, SCD gene, SNPsAbstract
This study examines the Stearoyl-CoA desaturase (SCD) gene, which is essential for converting saturated fatty acids (SFA) into monounsaturated fatty acids (MUFA). The research aims to analyze the diversity of Single Nucleotide Polymorphism (SNP) g.10428C>T within the SCD gene using the PCR-RFLP method. The investigation involved 40 blood samples from male Bali cattle aged 1.5 to 3 years, sourced from the Banjarmasin Slaughterhouse (RPH) in South Kalimantan. The SNP g.10428C>T diversity in the SCD gene was analyzed using the PCR-RFLP technique with the HinfI restriction enzyme. Genotype frequencies, allele frequencies, heterozygosity values, and the Hardy-Weinberg equilibrium were calculated using Popgen32. The relationship between fatty acid composition and the genotype of the SCD gene, as well as the fatty acids in Bali beef, was analyzed using a t-test. SCD|HinfI was found to have homozygous CC (0.03) and TT (0.85) and heterozygous CT (0.13) genotypes with a C allele frequency of 0.09 and T of 0.91. SNP g.10428C>T in the SCD gene was not significantly associated with fatty acid composition.
References
AlJohani, A. M., Syed, D. N., & Ntambi, J. M. (2017). Insights into Stearoyl-CoA desaturase-1 regulation of systemic metabolism. Trends in Endocrinology & Metabolism, 28(12), 831-842. https://doi.org/10.1016/j.tem.2017.10.003
Alwiyah, A., Nuraini, H., Agung, P. P., & Jakaria. (2016). Polymorphism Stearoyl-CoA Desaturase (SCD) gene and association with characteristics meat in Bali cattle. Trop Anim Sci J, 41, 188-195. https://doi.org/10.14710/jitaa.41.4.188-195
Anjalia, K. M., Mandala, A., Gunalang, B., Rubana, L., Anandajothia, E., Thineshsanthara, D., Manojkumara, T. G., & Kandan, S. (2019). Identification of six grouper species under the genus Epinephelus from Indian waters using PCR-RFLP of cytochrome c oxidase I (COI) gene fragment. J. Indonesian Trop. Anim. Agric., 101(1), 39-44. https://doi.org/10.1016/j.foodcont.2019.02.024
Batan, I. W., Fanggidae, B. C., Suatha, I. K., & Suarsana, I. N. (2018). Kepadatan dan kekuatan tulang sapi Bali betina yang dipelihara masyarakat di Bali (The density and strength of female Bali cattle bone rearing by Balinese community). Jurnal Veteriner, 19(3), 363-369. Retrieved from https://ojs.unud.ac.id/index.php/jvet/article/view/41682
Bhat, S. A., Ahmad, S. M., Ganai, N. A., Khan, S. M., Malik, A., Shah, R. A., & Iqbal, Z. (2017). Association of DGAT1, beta-casein and leptin gene polymorphism with milk quality and yield traits in Jersey and its cross with local Kashmiri cattle. J. Entomol. Zool. Stud., 5(6), 557–561. Retrieved from https://www.entomoljournal.com/archives/2017/vol5issue6/PartH/5-5-276-611.pdf
Dairoh, D., Jakaria, J., Ulum, M. F., & Sumantri, C. (2022). A New SNPs at 3’UTR Region of calpain 1 gene and its association with growth and meat quality traits in beef cattle. J. Indones. Trop. Anim. Agric., 47(1), 17-28. https://doi.org/10.14710/jitaa.47.1.17-28
Dairoh, Sutikno, Ishak, A. B., Priyatno, R., Sumatri, C., Ulum, M. F., & Jakaria. (2024). Fatty acid profiling of Bali and Wagyu cattle using principal component analysis. Bulletin of Animal Science, 48(1), 64-69. DOI:10.21059/buletinpeternak.v48i1.86454
Dao, W., Fan, X., Liang, J., Chen, T., Chang, Z., Zhang, Y., & Miao, Y. (2024). Molecular and Functional Analysis of the Stearoyl-CoA Desaturase (SCD) Gene in Buffalo: Implications for Milk Fat Synthesis. Animals, 14(23), 3191. https://doi.org/10.3390/ani14223191
Graffelman, J., Jain, D., & Weir, B. (2017). A genome-wide study of Hardy–Weinberg equilibrium with next generation sequence data. Hum. Genet., 136, 727–741. https://doi.org/10.1007/s00439-017-1786-7
Hafid, H., Hasnudi, H. A., Bain, F., Nasiu, Inderawati, P., Patriani, & Ananda, S. H. (2019). Effect of fasting time before slaughtering on body weight loss and carcass percentage of Bali cattle. IOP Conference Series: Earth and Environmental Science, 260, 012051. https://doi.org/10.1088/1755-1315/260/1/012051
Hilmia, N., Noor, R. R., Sumantri, C., Priyanto, R., & Gurnadi, E. (2017). Polymorphism of Stearoyl CoA Desaturase (SCD 1) gene in Indonesia local cattle. Journal of the Indonesia Tropical Animal Agriculture, 42(1), 1-5. DOI:10.14710/JITAA.42.1.1-5
Ismail, R., Handiwirawan, E., Elieser, S., & Jakaria, J. (2020). Polymorphism of 5’UTR myostatin gene indel (g.1256/TTTTA) and its association with body weight in Boerka crossbred goat. J. Indones. Trop. Anim. Agric., 45, 163-172. https://doi.org/10.14710/jitaa.45.3.163-172
Jakaria, H., Khasanah, H., Priyanto, R., Baihaqi, M. M., & Ulum, M. F. (2017). Prediction of meat quality in Bali cattle using ultrasound imaging. Journal of the Indonesian Tropical Animal Agriculture, 42(2), 59-65. https://doi.org/10.14710/jitaa.42.2.59-65
Jakaria, Ulum, M., Lestari, D., Akwila, S., Sihite, D. E. W. T., Priyanto, R., Muladno, & Sumantri, C. (2020). Investigating new SNPs of CAST, CAPN and SCD genes in 5’UTR of Bali cattle. Biodiversitas J Biol Divers, 21, 2971–2976. DOI:10.13057/biodiv/d210713
Karimah, S. N., Noor, R. R., & Jakaria, J. (2021). Identification of SNP g.10428 C > T of Stearoyl-CoA desaturase gene related to meat quality in Bali cattle using PCR-RFLP method. J. Indones. Trop. Anim. Agric., 46(4), 295-303. https://doi.org/10.14710/jitaa.46.4.295-303
Kubota, C. S., & Espenshade, P. J. (2022). Targeting Stearoyl-CoA Desaturase in Solid Tumors. Cancer Research, 82(9), 1682–1688. https://doi.org/10.1158/0008-5472.CAN-21-4044
Liu, M., Wei, Y., & Li, X. (2018). Quantitative phosphoproteomic analysis of caprine muscle with high and low meat quality. Meat Science, 141(11), 103-111. https://doi.org/10.1016/j.meatsci.2018.01.001
Liu, R., Fang, X., Lu, X., Liu, Y., Li, Y., Bai, X., Ding, X., & Yang, R. (2024). Polymorphisms of the SCD1 Gene and Its Association Analysis with Carcass, Meat Quality, Adipogenic Traits, Fatty Acid Composition, and Milk Production Traits in Cattle. Animals, 14, 1759. https://doi.org/10.3390/ani14121759
Martins, T. S., Lemos, M. V. A., Mueller, L. F., Baldi, F., Amorim, T. R., Ferrinho, A. M., Muñoz, J. A., Fuzikawa, I. H. S., Moura, G. V., Gemelli, J. L., & Pereira, A. S. C. (2018). Fat deposition, fatty acid composition, and its relationship with meat quality and human health. Meat Science and Nutrition, 17-37. https://doi.org/10.5772/intechopen.77994
Nogalski, Z., Przyby?ek, P. P., Szul, M. S., Nogalska, A., Kapitu?a, M. M., & Purwin, C. (2018). Carcass characteristics and meat quality of bulls and steers slaughtered at two different ages. Italian Journal of Animal Science, 17(2), 279-288. https://doi.org/10.1080/1828051X.2017.1383861
Pecina, M., & Ivankovic. (2021). Candidate genes and fatty acids in beef meat. Italian Journal of Animal Science, 20(1), 1716-1729. https://doi.org/10.1080/1828051X.2021.1991240
Wang, Z., Zhu, B., Niu, H., Zhang, W., Xu, L., Xu, L., Chen, Y., Zhang, L., Gao, X., & Gao, H. (2019). Genome-wide association study identifies SNPs associated with fatty acid composition in Chinese Wagyu cattle. J Anim Sci Biotechnol., 10(1), 1–13. https://doi.org/10.1186/s40104-019-0322-0
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