Pengaruh fermentasi kombinasi jamur Pleurotus ostreatus dengan Trichoderma viridae terhadap kandungan nutrien dan aktivitas enzim selulase bungkil kopra
Copra meal is by-product of oil extraction that is produced in large quantity and relatively cheap in Indonesia. However, its high cellulose becomes obstacle for poultry diet. Two studies was conducted to optimize the utilization of copra meal by producing crude enzyme that matched with copra meal using solid state fermentation method with various doses and incubation time that mixed with Pleurotus ostreatus (PO) and Trichoderma viridae (TV). In the first study, copra meal was fermented with 4 levels of inoculum (L0 = no inoculum; L1 = 17.7 CFU/g of TV and 175.00 CFU/g of PO per kg of copra meal; L2 = 35.4 CFU/g of TV and 218.75 CFU/g of PO per kg of copra meal; L3 = 53.1 CFU/g of TV and 262.50 CFU/g of PO per kg of copra meal and 4 incubation time (W1= 4 days; W2 = 6 days; W3 = 8 days; and W4 = 10 days). Parameters measured were crude protein, crude lipid, crude fibre and gross energy. A completely randomized factorial design was used in the study. In the second study, crude enzyme was produced from the best results found in the first study. A method of Jacob and Prema (2006) was used to produce enzyme. Meanwhile, activity of cellulase was measured based on the method of Omojosola (2008). The results showed that factor of inoculum level was found significantly increased protein content and gross energy but decreased crude lipid and crude fibre of the mixed fungi-fermented copra meal. Incubation time did not affect protein content but significanly affected crude lipid, crude fibre and gross energy. Interactions between inoculum level and incubation time was found in crude lipid, crude fibre and gross energy contents of mixed fungi-fermented copra meal. Activity of cellulase was 0.71 g glucose/l.
ÂKeywords : Fermentation, Pleurotus ostreatus, Trichoderma viridae, inoculum level, incubation time, cellulase activity
LicenseAuthors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).