Accuracy of early pregnancy diagnosis using interferon-tau (IFN-τ) in Aceh cows

Fisma Amri, Tongku Nizwan Siregar, Gholib Gholib, Husnurrizal Husnurrizal, Juli Melia, Budianto Panjaitan, Teuku Armansyah

Abstract


Interferon-tau (IFN-τ) is a pregnancy signal produced by embryonic trophoblast cells at the time of implantation in a mammal's endometrial wall, which is useful for conveying a message that the mammal can accept the presence of the foreign object (embryo). This study aims to determine the accuracy of early pregnancy diagnosis in Aceh cows by measuring the concentration of IFN-τ. This study involved eight cows aged 3-5 years, weighing 150-250 kg, clinically healthy, and having normal reproduction (have had calves and at least two regular cycles). All cows were estrous-synchronized twice using PGF2ɑ at a dose of 5 ml with 11 days intervals before artificial insemination (AI). Serum collection was performed on days 14, 15, 16, 17, 18 after AI. Based on ultrasound examination, out of eight cows, four (50%) cows were diagnosed as pregnant, and four (50%) cows were diagnosed as not pregnant. Mean (± SD) IFN-τ of pregnant vs. non-pregnant cows on day 14, 15, 16, 17, and 18 were 14.96±8.65 pg/ml vs. 6.14±5.54 pg/mL; 16.74±5.28 pg/mL vs. 4.44±3.51 pg/mL; 14.33±5.9 pg/mL vs. 5.78±5.20 pg/mL; 13.87±5.42 pg/mL vs. 4.38±3.76 pg/mL; and 13.93±6, 16 pg/mL vs. 3.24±2.63 pg/mL, respectively. The lowest IFN-τ concentration in cows that were successfully pregnant was 7.88±1.84 pg/mL. The best timing of IFN-τ for pregnancy diagnosis was on day 15 after AI, with an accuracy of 87.5%, specificity of 100%, and sensitivity of 80%.


Keywords


IFN-τ; Aceh cows; Diagnosis of pregnancy

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References


Amiruddin, Siregar, T. N., Riady, G., and Budiman, H. 2001. Efektivitas beberapa metode diagnosis kebuntingan pada sapi. Jurnal Medika Veterinaria, 1(2), 45-48.

Amrozi, and Setiawan, B. 2011. Sinkronisasi estrus dan pengamatan ultrasonografi pemeriksaan kebuntingan dini pada domba garut (Ovis aries) sebagai standar penentuan umur kebuntingan. Jurnal Kedokteran Hewan, 5(2): 73-77. doi: 10.21157/j.ked.hewan.v5i2.364

Antoniazzi, A. Q., Latimer, A. M., Romero, J.J., Niswender, G.D., Bazer, F.W., Nett, T.M., and Hansen, T.R. 2012. Detection of interferon-tau in uterine vein blood using a highly sensitive and specific radioimmunoassay. Biology of Reproduction, 87(1), 183-188. doi: 10.1093/biolreprod/87.s1.183

Balhara, A. K., Gupta, M., Singh, S., Mohanty, A. K., and Singh, I. 2013. Review article early pregnancy diagnosis in bovines: Current status and future directions. The Scientific World Journal. doi: 10.1155/2013/958540

Bazer, F. W., Spencer, T. E., Johnson, G. A., Burghardt, R. C., and Wu, G. 2009. Comparative aspects of implantation. Reproduction, 138(2), 195-209. doi: 10.1530/REP-09-0158.

Bott, R. C., Ashley, R. L., Henkes, L. E., Antoniazzi, A. Q., Bruemmer, J. E., Niswender, G. D., Bazer, F. W., Spencer, T. E., Smirnova, N. P., Anthony, R. V., and Hansen, T. R. 2010. Uterine vein infusion of interferon tau (IFN-τ) extends luteal life span in ewes. Biology of Reproduction, 82(4), 725-735. doi: 10.1095/biolreprod.109.079467.

Chaudary, A. K., and Purohit, G.N. 2012. Ultrasonographic detection of early pregnancy loss in dairy cows. Journal of Animal Science Advance, 2(8): 706-710.

Depamede, S. N. 2009. Peran interferon-tau (IFN-τ) dalam penanganan reproduksi ternak ruminansia betina. Wartazoa,19(4), 166-171.

Dorniak, P., Bazer, F. W., and Spencer, T. E. 2011. Prostaglandins regulate conceptus elongation and mediate effects of interferon tau on the ovine uterine endometrium. Biology of Reproduction, 84(6), 1119-1127. doi: 10.1095/biolreprod.110.089979.

Fleming, J-A. G. W., Choi, Y., Johnson, G. A., Spencer, T. E., and Bazer, F. W. 2001. Cloning of the ovine estrogen receptor-α promoter and functional regulation by ovine interferon-τ. Endocrinology, 142(7), 2879-2887, doi: org/10.1210/endo.142.7.8245

Hafizuddin, Yusmadi, and Anwar. 2016. Peran sitokin dan hormon selama proses maternal recognition of pregnancy pada ternak. Jurnal Ilmiah Peternakan, 4(1), 29-36.

Humblot, P. 2001. Use of pregnancy specific proteins and progesterone assays to monitor pregnancy and determine the timing, frequencies and sources of embryonic mortality in ruminants. heriogenology, 56(9), 1417-1433. doi: 10.1016/s0093-691x(01)00644-6.

Lee, J. M., Kim, H. S., Jeong, S. G., and Jung, J. K. 1996. Studies on early pregnancy determination in cows by using the enzyme-immunoassay and radio-immunoassay in milk. Asian-Australasian Journal of Animal Sciences, 9(3), 229-234. doi: 10.5713/ajas.1996.299

Lucy, M., and Poock, S. 2012. Pregnancy determination by palpation and beyond. Proceedings, Applied Reproductive Strategies in Beef Cattle December 3 - 4, Sioux Falls, SD

Nova, M.E., Riady, G., and Melia, J. 2014. Diagnosis kebuntingan dini menggunakan kit progesteron air susu pada kambing Peranakan Ettawah (Capra hircus). Jurnal Medika Veterinaria, 8(2), 120-124.

Sayuti, A., Melia, J., Marpaung, I. K., Siregar, T. N., Syafruddin, Amiruddin, and Panjaitan, B. 2016. Diagnosis kebuntingan dini pada kambing kacang (Capra sp.) menggunakan ultrasonografi transkutaneus. Jurnal Kedokteran Hewan, 10(1), 63-67. doi: org/10.21157/j.ked.hewan.v10i1.3373

Siregar, T. N. and Hamdan. 2008. Buku Ajar Teknologi Reproduksi pada Ternak. Fakultas Kedokteran Hewan Universitas Syiah Kuala, Banda Aceh.

Spencer, T. E. and Hansen, T.R. 2015. Implantation and establishment of pregnancy in ruminants. Advances in Anatomy, Embryology and Cell Biology, 216, 105-135. doi: 10.1007/978-3-319-15856-3_7.

Spencer, T. E., Forde, N., Dorniak, P., Hansen, T. R., Romero, J. J., and Lonergan, P. 2013. Conceptus-derived prostaglandins regulate gene expression in the endometrium prior to pregnancy recognition in ruminants. Reproduction, 146(4), 377-387. doi: 10.1530/REP-13-0165

Suprihatin, N., Tumbelaka, L. I., and Setiadi, M.A. 2016. Profil progesterone air susu dan tingkat kebuntingan sapi perah pascasinkroninasi estrus menggunakan prostaglandin F2Alfa atau progesterone-CIDR. Jurnal Veteriner, 17(3), 396-403. doi: 10.19087/jveteriner.2016.17.3.396

Wardani, M., Suyadi, and Nuryadi. 2013. Uji akurasi kebuntingan pada kambing menggunakan ultrasonography. http://fapet.ub.ac.id/wpcontent/uploads/2013/04/Uji- Akurasi-Kebuntingan-Pada-Kambing-Menggunakan Ultrasonography.pdf

Winkelman, G. L., Roberts, R. M., Peterson, A. J., Alexenko, A. P., and Ealy, A. D. 1999. Identification of the expressed forms of ovine interferon-tau in the periimplantation conceptus: Sequence relationships and comparative biological activities. Biology of Reproduction, 61(6), 1592-1600. doi: 10.1095/biolreprod61.6.1592




DOI: http://dx.doi.org/10.21776/ub.jiip.2021.031.03.06

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