The effect of age on semen freezability of Swamp Buffalo bull in a commercial artificial insemination center

Nurul Isnaini, Tri Harsi, Wahyu Riza Zamani


This study aimed to assess the effect of age on semen freezability (sperms motility before freezing, post-thawing, recovery rate, and straws production per ejaculate) of swamp buffalo bull kept in Lembang Artificial Insemination Center. A total of 115 ejaculates collected from one swamp buffalo bull at 5, 6 and 7 years old were included in the study. The results showed that the overall mean values of sperms motility before freezing, post-thawing, recovery rate, and straws production per ejaculate of swamp buffalo bull were 62.13%, 43.61%, 62.16%, and 114.16 doses, respectively. The age of bull did not show significant (P>0.05) effect on sperms motility before freezing, however the sperms motility post-thawing, recovery rate, and straws production per ejaculate was influenced (P<0.01) by bull age. At seven years old, the poorest (P<0.01) post-thawing sperms motility and recovery rate were noted in comparison to 5 and 6 years old, nevertheless the difference in post-thawing sperms motility was only less than 3% point, which was not biologically important. On the other hand, the semen collection at seven years old had a higher (P<0.01) straws production per ejaculate than those at 5 and 6 years old (133.70 vs. 106.39 and 90.61 doses, respectively). In conclusion, this study provides evidence that the semen freezability of swamp buffalo bull is varied among the age with the peak frozen semen production was achieved at seven years old.


artificial insemination; Bubalus bubalis; bull age; sperms motility; frozen semen production

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Ahmed, S., Khan, M. I. ur R., Ahmad, M., & Iqbal, S. (2018). Effect of age on lipid peroxidation of fresh and frozen-thawed semen of Nili-Ravi buffalo bulls. Italian Journal of Animal Science, 17(3), 730–735. https://doi.o rg/10.1080/1828051X.2018.1424569

Amin, M. R., Toelihere, M. R., Yusuf, T. L., & Situmorang, P. (1999). Pengaruh plasma semen sapi terhadap kualitas semen beku kerbau lumpur (Bubalus bubalis) (in Bahasa Indonesia). Jurnal Ilmu Ternak Dan Veteriner, 4, 143–147.

Baruselli, P. S., Ferreira, R. M., Sá Filho, M. F., & Bó, G. A. (2018, June 1). Review: Using artificial insemination v. natural service in beef herds. Animal. Cambridge University Press.

Bhakat, M., Mohanty, T. K., Raina, V. S., Gupta, A. K., Khan, H. M., Mahapatra, R. K., & Sarkar, M. (2011). Effect of age and season on semen quality parameters in Sahiwal bulls. Tropical Animal Health and Production, 43(6), 1161–1168. https://

Central Data and Information System of Agriculture. (2017). Outlook Daging Sapi (in Bahasa Indonesia). Jakarta: Ministry of Agriculture Republic of Indonesia.

Deka, R., Nath, K. C., Bhuyan, M., Bhuyan, D., Das, G. C., Dutta, L., & Borpujari, D. (2019). Parturition behavior of Swamp buffalo cows (Bubalus bubalis) under organized system of rearing. Biological Rhythm Research.

Directorate General of Livestock and Animal Health. (2014). Livestock and Animal Health Statistics. Jakarta: Ministry of Agriculture Republic of Indonesia.

Directorate General of Livestock and Animal Health. (2018). Livestock and Animal Health Statistics. Jakarta: Ministry of Agriculture Republic of Indonesia.

El Sisy, G. A., Abo El-Maaty, A. M., & Rawash, Z. M. (2016). Comparative blood and seminal plasma oxidant/antioxidant status of Arab stallions with different ages and their relation to semen quality. Asian Pacific Journal of Reproduction, 5(5), 428–433. jr.2016.07.006

Islam, M., Apu, A., Hoque, S. A., Ali, M., & Karmaker, S. (2018). Comparative study on the libido, semen quality and fertility of Brahman cross, Holstein Friesian cross and Red Chittagong breeding bulls. Bangladesh Journal of Animal Science, 47(2), 61–67. https://

Isnaini, N., Wahjuningsih, S., & Adhitama, E. (2019). Seasonal effects on semen quality of Ongole crossbred and Simmental bulls used for artificial insemination. Livestock Research for Rural Development.

Isnaini, N., Wahjuningsih, S., Ma’ruf, A., & Witayanto, D. A. (2019). Effects of age and breed on semen quality of beef bull sires in an Indonesian artificial insemination center. Livestock Research for Rural Development.

Kumar, A., Singh, P., Bhakat, M., Singh, S., Nitharwal, K., & Gupta, A. K. (2017). Effect of feed energy levels on semen quality and freezability of young murrah buffalo bulls. Buffalo Bulletin, 36(2), 415–426.

Murphy, E. M., Kelly, A. K., O’Meara, C., Eivers, B., Lonergan, P., & Fair, S. (2018). Influence of bull age, ejaculate number, and season of collection on semen production and sperm motility parameters in holstein friesian bulls in a commercial artificial insemination centre. Journal of Animal Science, 96(6), 2408–2418.

Naveena, B. M., & Kiran, M. (2014). Buffalo meat quality, composition, and processing characteristics: Contribution to the global economy and nutritional security. Animal Frontiers, 4(4), 18–24. https://doi. org/10.2527/af.2014-0029

Pant, H. C., Sharma, R. K., Patel, S. H., Shukla, H. R., Mittal, A. K., Kasiraj, R., Prabhakar, J. H. (2003). Testicular development and its relationship to semen production in Murrah buffalo bulls. Theriogenology, 60(1), 27–34. 02)01037-3

Snoj, T., Kobal, S., & Majdic, G. (2013). Effects of season, Age, And breed on semen characteristics in different Bos taurus breeds in a 31-year retrospective study. Theriogenology, 79(5), 847–852. 16/j.theriogenology.2012.12.014

Susilawati, T., Ratnawati, D., Isnaini, N., Kuswati, K., & Yekti, A. P. (2018). Character of liquid semen motility in various diluents on Balinese cattle during cold storage. Asian Journal of Microbiology, Biotechnology and Environmental Sciences, 20(1), 166–172.



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