Study on the Marsilea crenata extract supplementation on caudal epididymal plasma (CEP-3) to the Boer semen quality during cold storage

Authors

  • Sri Wahjuningsih Faculty of Animal Science, University of Brawijaya, Malang
  • Desinta Wulandari Faculty of Animal Science, University of Brawijaya, Malang

DOI:

https://doi.org/10.21776/ub.jiip.2019.029.03.08

Keywords:

CEP-3, antioxidant, semen quality, water clover extract

Abstract

The success of artificial insemination (AI) would be determined by the semen quality during storage. This research aims to evaluate the quality of Boer goat liquid semen stored with caudal epididymal plasma (CEP-3) supplemented with water clover (Marsilea crenata) extract or WCE during cold storage (3-5ºC). The semen was collected from Boer goat aged 2.5 to 3 years old by using an artificial vagina. The semen with >2+ mass motility and >70% individual motility were then selected for the study. A randomized block design which consisted of four treatments with ten replications as follow: P0 or control (CEP-3 + 10% egg yolk + 0.4% albumen); P1 (CEP-3 + 10% egg yolk + 0.4% albumen + 1% WCE; P2 (CEP-3 + 10% egg yolk + 0.4% albumen + 2% WCE); P3 (CEP-3 + 10% egg yolk + 0.4% albumen + 3% WCE) was used in this study. The data were analyzed with ANOVA and followed with Duncan’s multiple range test to determine significant differences. The results of this study showed that motility, viability, and membrane integrity in all treatments showed highly significant differences (P<0.01), while no significant difference (P>0.05) was shown on the semen abnormality. The result concludes that 1% WCE supplementation to the CEP-3 extender showed the best Boer semen quality during 96-hours cold storage. 

References

Ax, R., M. Dally., B. Didion., R. Lenz., C. Love., D. Varner., E.S.E. Hafez. and M. Bellin. 2008. Semen Evaluation. In Reproduction in Farm Animal. Hafez, E.S.E. (Ed). 7th Edition. Lea and Febiger, USA.

Costa, N., T. Susilawati., N. Isnaini. dan M. Ihsan. 2016. Effect of Different Dilution Materials Usage on Indonesian Peranakan Ongole Bull Sperm Quality During Cooling Process. Indo American Journal of Pharmaceutical Sciences. 3 (4): 379-385.

Daniel, R.M., Stelian, dan Dragomir. 2010. The Effect of Acute Physical Exercise on The Antioxidant Status of The Skeletal and Cardiac Muscle in The Wistar Rat. Romanian Biotechnological Letters. 15 (3): 56-61.

Ditjennak. 2017. Statistik Peternakan dan Kesehatan Hewan 2017. Direktorat Jenderal Peternakan dan Kesehatan Hewan Kementerian Pertanian RI. ISBN: 978-979-628-034-6.

Ducha N, T. Susilawati, Aulanniam, S. Wahyuningsih, M. Pangestu. 2012 Ultrastructure and Fertilizing Ability of Limousin Bull Sperm after storage in Cep-2 extender with and without Egg Yolk. Pakistan Journal of Biological Sciences, 15 (20):979-985.

Feradis. 2010. Peranan Antioksidan dalam Pembekuan Semen. Jurnal Peternakan. 6 (2): 63-70.

Istanty, A.S., M.A. Salim., N. Isnaini. dan T. Susilawati. 2017. Pengaruh Penggantian Bovine Serum Albumin (BSA) Dengan Putih Telur Dalam Pengencer Dasar CEP-2 Terhadap Kualitas Semen Kambing Boer pada Simpan Dingin. J. Ternak Tropika. 18(1): 1-9.

Khaira, K. 2010. Menangkal Radikal Bebas dengan Antioksidan. Jurnal Sainstek. 11 (2): 183-187.

Latief, M., F. Tafzi, dan A. Saputra. 2013. Aktivitas Antioksidan Ekstrak Metanol Beberapa Bagian Tanaman Kayu Manis (Cinnamomum burmani) Asal Kabupaten Kerinci Provinsi Jambi. Prosiding Semirata FMIPA Universitas Lampung. 233-236.

Lubis, T.M., Dasrul, C.N. Thasmi. dan Akbar. 2013. Efektivitas Penambahan Vitamin C dalam Pengencer Susu Skim Kuning Telur terhadap Kualitas Spermatozoa Kambing Boer Setelah Penyimpanan Dingin. Jurnal Sains Pertanian. 3 (1): 347-361.

Lukman H Y, W.Busono, S.Wahjuningsih and Suyadi. 2014. Sperm motility and viability after α-tocopherol dilution in Tris aminomethane-base extender during cold storage in bali bull. International Journal of ChemTech Research, 6 (14): 5726-5732.

Nurjannah, A. Azka, dan A. Abdullah. 2012. Aktivitas Antioksidan dan Komponen Bioaktif Semanggi Air (Marsilea crenata). Jurnal Inovasi dan Kewirausahaan. 1 (3): 151-158.

Pereira, G. R., E. G. Becker, L. C. Siquera, R. Ferreira,C. K. Savero, V. S. Truzzi, Oliveira and Goncalves. 2010. Assessment of Bovine Spermatozoa Viability Using Different Cooling Protocols Prior to Cryopresertion. Italian Journal of Animal Sciene. 9(88): 464-470.

Rochim, A., M.A. Salim, N. Isnaini, dan T. Susilawati. 2017. Pengaruh Penghilangan Rafinosa dalam Pengencer Tris Aminomethane Kuning Telur Terhadap Kualitas Semen Kambing Boer Selama Simpan Dingin. Jurnal Ternak Tropika. 18 (1): 27-35.

Saefudin, S. Marusin, dan Chairul. 2013. Aktivitas Antioksidan pada Enam Jenis Tumbuhan Sterculiaceae. Jurnal Penelitian Hasil Hutan. 31 (2): 103-109.

Sudarmanto, I., dan T. Suhartati. 2015. Aktivitas Antioksidan Senyawa Flavonoid pada Kulit Akar Tanaman Ara (Ficus racemosa, L). Jurnal Kesehatan. 6 (2): 137-141.

Susilawati, T., N. Isnaini, A.P.A. Yekti, I. Nurjannah, Errico, dan N. da Costa. 2016. Keberhasilan Inseminasi Buatan Menggunakan Semen Beku dan Semen Cair pada Sapi Pernakan Ongole. Jurnal Ilmu-Ilmu Peternakan. 26 (3):14-19.

Suyadi., A. Rachmawati. dan N. Iswanto. 2017. Pengaruh α-Tocopherol yang Berbeda Dalam Pengencer Dasar Tris Aminomethan Kuning Telur Terhadap Kualitas Semen Kambing Boer yang Disimpan pada Suhu 5ºC. Jurnal Ilmu-Ilmu Peternakan. 22(3): 1-8.

Verberckmoes, S., A. Van Soom, J. Dewulf, I. De Pauw, and A. De Kruif. 2004. Storage of fresh bovine semen in diluent based on the ionic composition cauda epididymal plasma. J. Reprod.Dom. Anim. 39(6):1-7.

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Published

2019-12-06

How to Cite

Wahjuningsih, S., & Wulandari, D. (2019). Study on the Marsilea crenata extract supplementation on caudal epididymal plasma (CEP-3) to the Boer semen quality during cold storage. Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science), 29(3), 259–265. https://doi.org/10.21776/ub.jiip.2019.029.03.08

Issue

Vol. 29 No. 3 (2019): Desember

Section

Articles

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