Supplementation of black soldier fly (Hermetia illucens) on productivity and blood hematology

Andri Cahya Irawan, Dewi Apri Astuti, I Wayan Teguh Wibawan, Widya Hermana


Black Soldier Fly larvae are natural antibiotics. The use of methanol extract in BSF larvae has a dual function, namely high protein content and high antibiotic content to kill Gram-negative bacteria. The biological structure of BSF larvae with antimicrobial peptide (AMP) acts as an inhibitor of pathogenic microorganisms, has a high lauric acid function as a natural antimicrobial and chitin, the polysaccharide plays a role in enhancing the immune response of the animal. This study aims to gain the best type of BSF protein from feed treatments containing live BSF, dry BSF, and BSF extract to increase blood productivity and hematology. The results of this study show that the best P3 treatment for the productivity of laying hens at the age of 18 to 26 weeks with the use of BSF extract since it contains the highest protein compared to other treatments. Productivity variables show the best P3 treatment, each egg weight 57.17 g bird-1, daily egg production 90.88%, egg mass 2408.16 g bird-1 and ration conversion 2.0. The mean hematology of the blood showed the highest P3 treatment, each erythrocyte 2.91±0.13 x 106 mm-3, leucocytes 17.69±3.68 x 103 mm-3, hematocrit 27.8±1.14%, hemoglobin 9.96±1.12%, MCV 95.77±4.41 fl, MCHC 35.99±5.21%, MCH 34.38±4.53 pg, lymphocytes 61.2 ± 4.86%, heterophile 31.2±2%, monocytes 5±0.62% and ratio H/L 0.51±0.04.


black Soldier Fly (BSF); antimicrobial peptide (AMP); productivity; and hematology.

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Altan, O., Altan, A., Cabuk, M., & Bayraktar, H. (2000). Effects of heat stress on some blood parameters in broilers. Turkish Journal of Veterinary and Animal Sciences , 24(2), 145–148.

Altan, Ö., Pabuçcuoğlu, A., Altan, A., Konyalioğlu, S., & Bayraktar, H. (2003). Effect of heat stress on oxidative stress, lipid peroxidation and some stress parameters in broilers. British Poultry Science, 44(4), 545–550. 80/00071660310001618334

Amrullah, I. (2004). Nutrisi Ayam Petelur. Bogor: Lembaga Satu Gunungbudi.

Barragan-Fonseca, K., Dicke, M., & van Loon, J. J. (2017). Nutritional value of the black soldier fly ( Hermetia illucens L.) and its suitability as animal feed – a review. Journal of Insects as Food and Feed, 3(2), 105–120. 0055

Barros-Cordeiro, K. B., Báo, S. N., & Pujol-Luz, J. R. (2014). Intra-puparial development of the black soldier-fly,Hermetia illucens. Journal of Insect Science, 14(1), 1–10. https://

Belluco, S., Losasso, C., Maggioletti, M., Alonzi, C. C., Paoletti, M. G., & Ricci, A. (2013). Edible insects in a food safety and nutritional perspective: a critical review. Comprehensive Reviews in Food Science and Food Safety, 12(3), 296–313. 7.12014

Bird, N., Huton, P., Morrison, W., & Weber, L. (2003). Heat Stress in Caged Layers. Ontario: Ministry of Agriculture and Food.

Blecha, F. (2000). Immune System Response to Stress. (G. Moberg & J. Mench, Eds.). New York: Cabi Publishing.

Bosch, G., Zhang, S., Oonincx, D. G. A. B., & Hendriks, W. H. (2014). Protein quality of insects as potential ingredients for dog and cat foods. Journal of Nutritional Science, 3, 1–4.

Bovera, F., Piccolo, G., Gasco, L., Marono, S., Loponte, R., Vassalotti, G., Nizza, A. (2015). Yellow mealworm larvae ( Tenebrio molitor , L.) as a possible alternative to soybean meal in broiler diets. British Poultry Science, 56(5), 1–7. 10.1080/00071668.2015.1080815

Campbell, T. (2015). Exotic Animal Hematology and Cytology (4th ed.). Oxford: John Wiley & Sons, inc.

Campbell, T., Mary, A., & Glade, W. (2012). Veterinary Hematology and clinical chemistry (2nd ed.). Colorado: Fort Collins.

Cayan, H., & Erener, G. (2015). Effect of olive leaf (Olea europaea) powder on laying hens performance, egg quality and egg yolk cholesterol levels. Asian-Australasian Journal of Animal Sciences, 28(4), 538–543. https://doi. org/10.5713/ajas.14.0369

Challenger, W. O., Williams, T. D., Christians, J. K., & Vézina, F. (2001). Follicular development and plasma yolk precursor dynamics through the laying cycle in the european starling ( Sturnus vulgaris ). Physiological and Biochemical Zoology, 74(3), 356–365.

Choi, W.-H., Yun, J.-H., Chu, J.-P., & Chu, K.-B. (2012). Antibacterial effect of extracts of Hermetia illucens (Diptera: Stratiomyidae) larvae against Gram-negative bacteria. Entomological Research, 42(5), 219–226. https://doi. org/10.1111/j.17485967.2012.00465.x

Damayanti, R., Lusiana, N., & Prasetyo, J. (2017). Studi pengaruh ukuran partikel dan penambahan perekat tapioka terhadap karakteristik biopelet dari kulit coklat (Theobroma Cacao L.) sebagai bahan bakar alternatif terbarukan. Jurnal Teknotan, 11(1), 51–60. https://doi. org/10.24198/jt.vol11n1.6

Davis, A. K., Maney, D. L., & Maerz, J. C. (2008). The use of leukocyte profiles to measure stress in vertebrates: a review for ecologists. Functional Ecology, 22(5), 760–772. https://doi.or g/10.1111/j.1365-2435.2008.01467.x

Dellman, H., & Brown, E. (1992). Histologi Veteriner. (R. Hartono, Ed.). Jakarta: Universitas Indonesia Press.

Dierenfeld, E. S., & King, J. (2008). Digestibility and mineral availability of phoenix worms, hermetia illucens, ingested by mountain chicken frogs, leptodactylus fallax. Journal of

Herpetological Medicine and Surgery, 18(3), 100–105. https://doi. org/10.5818/1529-9651.18.3-4.100

Djulardi, A., Muis, H., & Latif, S. A. (2006). Nutrisi Aneka Ternak dan Satwa Harapan. Padang: Universitas Andalas.

Finke, M. D. (2013). Complete Nutrient content of four species of feeder insects. Zoo Biology, 32(1), 27–36.

Fischbach, F., & Marshall, B. D. (2009). A Manual of Laboratory and Diagnostic (8th ed.). Philadelphia: Williams & Wilkins.

Frandson, R. (1992). Anatomi dan Fisiolgi Ternak. (B. Srigandono & P. Koen, Eds.) (4th ed.). Yogyakarta: Gadjah Mada University Press.

Ganong, E. (2008). Buku Ajar Fisiologi Kedokternan (Review of Medical Physiology) (2nd ed.). Jakarta: Penerbit Buku Kedokteran EGC.

Gillespie, J., & Flanders, F. (2010). Modern Livestock and Poultry Production (8th ed.). New York: Delmar Cengage Learning.

Gutiérrez, G. P. A., Ruiz, R. A. V., & Vélez, H. M. (2004). Compositional, microbiological and protein digestibility analysis of larval meal of Hermetia illucens (Diptera:Stratiomyiidae) at Angelópolis-Antioquia, Colombia. Revista - Facultad Nacional de Agronomía Medellín, 57(2), 2491–2499.

Guyton, A., & Hall, J. (2010). Textbook of Medical Physiology (8th ed.). Missisipi: Elsevier Inc.

Hamzah, R., Wiryanti, I., Astuti, D., & Satrija, F. (2014). Tanggap kebal dan tampilan produksi ayam pedaging yang diberi ekstrak buah mengkudu. Jurnal Veteriner, 13(1), 34–42.

Harlystiarini, H., Mutia, R., Wibawan, I. W. T., & Astuti, D. A. (2019). In vitro antibacterial activity of black soldier fly (hermetia illucens) larva extracts against gram-negative bacteria. Buletin Peternakan, 43(2), 125–129. etinpeternak.v43i2.42833

Hendrix Genetic Company. (2015). Isa Brown Management Guide. Peterborough: Hendrix Genetic Company.

Heuzé, V., Tran, G., & Kaushik, S. (2015). Fish meal. Feedipedia, program dari INRA, CIRAD, AFZ and FAO.

Khan, C. (2005). The Merk Veterinary Manual (9th ed.). Philadelphia: Nutritional Publishing.

Kim, S. A., & Rhee, M. S. (2016). Highly enhanced bactericidal effects of medium chain fatty acids (caprylic, capric, and lauric acid) combined with edible plant essential oils (carvacrol, eugenol, β-resorcylic acid, trans -cinnamaldehyde, thymol, and vanillin) against Escherichia coli O1. Food Control, 60, 447–454.

Lesson, S., & Summer, J. D. (2005). Comercial Poultry Nutrition (3rd ed.). Canada: University Guelph.

Mashaly, M. M., Hendricks, G. L., Kalama, M. A., Gehad, A. E., Abbas, A. O., & Patterson, P. H. (2004). Effect of heat stress on production parameters and immune responses of commercial laying hens. Poultry Science, 83(6), 889–894. 3.6.889

Mattjik, A., & Sumertajaya, M. (2002). Perancangan Percobaan dengan Aplikasi SAS dan Minitab (2nd ed.). Bogor: IPB Press.

McBeath, W. (1992). Compendium for the Microbiological Examinations of Foods (3rd ed.). USA: American Public Health Association.

McDonald, P., Edwards, R., Greenhalgh, J. F., Morgan, C., Sin



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