Aktivitas antimikroba dan antioksidan ekstrak tepung daun dan buah mengkudu (Morinda citrifolia)
DOI:
https://doi.org/10.21776/ub.jiip.2018.028.02.02Keywords:
Noni fruit, Total phenol, DPPH test, Inhibition zone diameterAbstract
Noni fruit is a medical plant with active compound like antimicrobial and antioxidant that could potentially be used as a feed additive in poultry. This study aimed to determine the activity antimicrobial and antioxidant of noni fuit leave and fruit extract. The method was field experiment arranged in Completely Randomize Design with 3 treatments and 3 replications. The treatments are metanol extract of noni fruit powder, leave noni powder and combination noni fruit and leave powder. The inhibition zone diameter was determined by disc diffusion method, total phenol was determined by Folin-Ciocalteu method, Flavonoid was determined by Aluminium clorida method and antioxidant activity by DPPH (1,1-Diphenyl-2picrylhydrazyl) method. The results showed that noni powder ex-tract had significantly effect (P<0.01) in inhibition of Lactic Acid Bacteria and Escherichia coli. The total phenol of Noni fruit powder is highest approximately 1.67 mg/g GAE. The flavonoid of Noni leave is highest approximately 0.0438 mg/g QE. The DPPH test of Noni fruit is highest approximately 84.03%. It can be concluded that noni fruit extract can be used as an antibacterial and antioxidant in poultry.References
Assanga S.B.I., Lewis L.L.M., Rivera C.E.G. Gil-Salido, A.A. Acosta-Silva A.L., Meza-Cueto C.Y. and Rubio-Pino J.L. 2013. Effect of Maturity and Harvest Season on Antioxidant Activity, Phenolic Compounds and Ascorbic Acid of Morinda citrifolia L. (noni) Grown in Mexico. African Journal of Biotechnology, 12(29), 4630-4639.
Assi, R.A., Y. Darwis, Ibrahim M.A., Ar-shad A.K, L. Vuanghao and M.H. Laghari. 2015. Morinda citrifolia (Noni): A Comprehensive Review on Its Industrial Uses, Pharmacological Activities, and Clinical Trials. Ara-bian Journal of Chemistry, 10(5),: 691-707.
Blanco Y.C., F. Vaillant, A.M. Perez, M Reynes, J.M. Brillouet and P. Brat. 2006. The Noni Fruit: A Review of Agriculture Research, Nutritional and Teurapeutic Properties. Journal of Food Composition and Analysis, 19(6-7), 645-654.
Damayanti, E. dan T.B. Suparjana. 2007. Efek Penghambatan Beberapa Fraksi Ekstrak Buah Mengkudu terhadap Shingella dysenteriae. Prosiding Seminar Nasional Teknik Kimia Ke-juangan. Fakultas Biologi Universi-tas Jendral Soedirman. Yogyakarta.
Deng, S., West B.J., and Jensen C.J. 2010. A Quantitative Comparison of Phy-tochemical Components in Global Noni Fruits and Their Commercial Products. Food Chemistry, 122(1), 267-270.
Kaur S. and P. Mondal. 2014. Study of To-tal Phenol and Flavonoid Content, Antioxidant Activity and Antimicro-bial Properties of Medicinal Plants. Journal of Microbiology and Exper-imentation 1(1): 00005.
Krishnaiah, D., Nithyanandam, R., & Sarbatly, R. 2012. Phytochemical Constituents and Activities of Morinda citrifolia L. Dalam V. Rao (Ed.), Phytochemicals. Rijeka: Intech Open. https://doi.org/10.5772/26094
Kumar, S.N.K., M. Suresh, S.A. Kumar and P. Kalaiselvi. 2014. Bioactive Compounds, Radical Scavering, An-tioxidant Properties and FTIR Spec-troscopy Study of Morinda clitrifo-lia Fruit Extracts. International Journal of Current Microbiology and Applied Sciences, 3(2), 28-42.
Zin, Z. M., Abdul-Hamid, A., & Osman, A. (2002). Antioxidative activity of extracts from Mengkudu (Morinda citrifolia L.) root, fruit and leaf. Food Chemistry, 78(2), 227–231.
Oliver, S.P., B.E. Gillespie, M.J. Lewis, S.J. Ivey, R.A. Almeida, D.A. Moorrehead and H.H. Dowlen. 2001. Efficiency of a New Premilking, Teat Disinfectant Containing a Phe-nolic Combination for The Preven-tion of Mastitis. Journal Diary Sci-ence, 84, 1545-1549.
Pelezar M.J. dan E.C.S. Chan. 1988. Da-sar-Dasar Mikrobiologi. Terjema-han. Universitas Indonesia Press. Ja-karta.
Purwantiningsih, Suranindyah dan Wido-do. 2014. Aktivitas Senyawa Fenol dalam Buah Mengkudu (Morinda citrifolia) sebagai Antibakteri Alami untuk Penghambatan Bakteri Penyebab Mastitis. Buletin Peter-nakan, 38(1), 59-64.
Pangestuti, I. E., Sumardianto, dan Ama-lia, U. 2017. Skrining Senyawa Fitokimia Rumput Laut Sargassum Sp. dan Aktivitasnya Sebagai Anti-bakteri Terhadap Staphylococcus au-reus dan Eschericia coli. Ijfst, 12(2), 98–102.
Singh, D.R. 2012. Morinda citrifolia L: A Review of the Scientific Validation for Its Nutritional and Therapeutic Properties. Journal of Diabetes and Endocrinology, 3(6), 77-91.
Downloads
Published
Issue
Section
License
Authors who publish with this journal agree to the following terms:- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
