Fertilitas Spermatozoa Ayam Buras dengan Penambahan Antioksidan Glutathione dalam Pengencer Ringer’s Selama Simpan Dingin

iswati iswati, Nurul Isnaini, Trinil Susilawati


Testing of spermatozoa fertility in vivo is needed to find out the extent of the ability
of spermatozoa fertilization in the female reproductive tract. The fertility ability of
spermatozoa in fertilizing the ovum is indicated by the number of fertile eggs from
the number of eggs incubated resulting from the mating with Artificial Insemination
(AI). The aim of this research is to know the percentage of fertility of eggs from AI
using semen which has been stored cold for 8 hours on Ringe's diluent with the
addition of antioxidant glutathione. Collection semen with teaser female method is
done in Poultry Breeder Installation of Sekolah Tinggi Penyuluhan Pertanian (STPP)
Malang. The 30 female chickens were divided into 3 groups of treatment P0: AI
using fresh semen with Ringer's diluent, P1: AI using cold cement with Ringer's
Diluent (8 hours duration storage) and P2: AI using cold semen with 0.5 mM
addition in Diluent Ringe's (8 hours duration storage). Experiment design using
Complete Randomized Design (RAL) and  nonparametric data analysis with chi-square.
The observed variable was egg fertility calculated based on egg fertility percentage.
The results showed that the dilution treatment had a significant effect (P <0,05) on
fertility egg, with the best result indicated by P0 that is 76% fertility, followed
by P2 with fertility 42% and P1 which showed the lowest fertility was 34%. IB with
cold cement has not demonstrated a good percentage of fertility despite 0.05 mM
glutathione added as an antioxidant. It is recommended that AI in domestic poultry
using fresh semen with Ringe's diluent, is done as soon as possible to obtain optimum


fertility;domestic chicken;Artificial Insemination;glutathione;teaser female

Full Text:



Alkan, S., A. Baran, O.B. Ozdas dan, M. Evecen 2002. Morfological defects in turkey semen.Turk.J.Vet. Animal Science. 26:1087-1092.

Chelmonska, B., A. Jerysz, E. L Ukaszewicz, A. Kowalczyk, I. Malecki. 2008. Semen Collection from Japanese Quail (Coturnix japonica) Using a Teaser Female.Turk.J. Vet. Anim. Sci.32(1): 19-24

Danang, D.R, N. Isnaini dan P.Trisunuwati. 2012. Pengaruh Lama Simpan Semen Terhadap Kualitas Spermatozoa Ayam Kampung Dalam Pengencer Ringer’s pada suhu 4°C.Jurnal Ternak Tropika . 13 (1): 47-57

Donoghue. A.M., and G.J.Wishart (2000). Storage of poultry semen. Animal Reproductive Science, 62: 213-232.

Getachew, T. 2016. A Review Article of Artificial Insemination in Poultry. World’s Veterinary Journal. 6 (1):26-35.

Habibullah, M., M. A. Hashem, M. S. Rana and M. H. Islam. 2015. Effect of Artificial Insemination on different production parameter in Hubbard classic broiler parent stock. J. Bangladesh Agril. Univ. 13(1): 71–77.

Hopkins, S.M. and L.E.Evans. 2003. Artificial Inseminations in McDonald’s Veterinary Endocrinology and Reproduction.Edited by Mauricho H. Pineda and Michael P.Dooley. 5thedition. Iowa statePress. USA: 366-368.

Irastuti. 2011. Pengaruh Bangsa Pejantan dan Interval Deposisi Sementerhadap Keberhasilan Inseminasi Buatan pada ayam. Jurnal Sains dan Teknologi Tadulako. 1(1): 13-25.

Khan, R.U. 2011. Antioxidant and Poultry Semen Quality.World’s Poultry Science Journal.67: 298-308.

Maia, M.S., S.D. Bicudo, H.C. Azevedo, C.C. Sicherle, D.B. Sousa, and L. Rodello. 2009. Motility and Viability of Ram Sperm Cryopreserved in a Tris-Egg Yolk Extender Supplemented with Anti-Oxidants. Small Ruminant Research. 85: 85-90.

Malecki, I., and G.B Martin. 2002. Semen collection in the emu and ostrich.Proceedings of the World Ostrich Congress. Warsaw, Poland: 38-43.

Modupe, O., A. C Livinus and N.B. Ifeany. 2013. Semen Quality Characteristic and Effect of Mating Ratio on Reproductive Performance of Hubbarrd Broiller Bredders. Journal of Agriculture Science. 5(1) 154-159.

Ridwan dan Rusdin. 2008. Konservasi Semen Ayam Buras Menggunakan Berbagai Pengencer Terhadap Fertilitas Dan Periode Fertil Spermatozoa Pasca Inseminasi Buatan.J. Agroland 15 (1) : 63 – 67.

Saleh D.M., dan A.Y. Isyanto . 2011. Pengaruh Lama Penyimpanan terhadap Motilitas dan Fertilitas Spermatozoa Ayam Kate Lokal. Ckrawala Galuh. 1(6): 1-6.

Sanocka, D.M. and Kurpisz, M. 2004. Reactive Oxygen Species and Sperm Cells. Reproductive Biology and Endocrinology. 2: 12-18.

Solihati, N., A. Pinnezak R. Idi, R. Setiawan, I.Y. Asmara, B.I. Sujana., 2006. Pengaruh Lama PenyimpananSemen Cair Ayam Buras pada Suhu 5°C terhadap Periode Fertil dan Fertilitas Sperma.Jurnal Ilmu ternak. 6(1):7-11

Surai, P.F., N. Fujihara, B.K. Speake, J-P.Brillard, G.J. Wishart and N.H.C.Sparks. 2001. Polyunsaturated Fatty Acids, Lipid Peroxidation and Antioxidant Protection in Avian semen. Review. Asian-Aust,J.Animal science. 14 (7): 1024-1050.

Tabatabaei, S. 2010. The effec Spermatozoa Number on Fertility Rate of Chicken in Artificial Insemination Programs. Journal of Animal and Veterinary Advances. 9(12): 1717-1719.

Thananurak, P.,C. Sittikasamkit, T. Vongpralub and K. Sakwiwatkul. 2015. Effects of Addition of Reduced Glutathione to Thawing media on Motility Parameters, Lipid Peroxidation and Fertility Rate in Frozen-Thawed Chicken Spermatozoa. Khon Kaen Agr Journal. 43 (suplement 2): 98-102.

Thuwanut, P., K. Chatdarong, A.S Bergqvist, L. Söderquist, K. Thiangtum, D Tongthainan, and E. Axnér. 2011. The Effects of Antioxidants on Semen Traits and in Vitro Fertilizing Ability of Sperm from Flat-Headed Cat (Prionailurus planiceps). Theriogenology. 76: 115-125.

Zahariev Z., R. Stefanof., M. Sabev., K. Miteva., and I. Nikolav.,2007. Cytochrome Affect Viability and Fertility of Bull Semen, am-Euras. J. Agric and Environ.2(1): 48-50

DOI: http://dx.doi.org/10.21776/ub.jiip.2017.027.01.10


  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.