Carcass yield and health status of broilers fed aflatoxin B1 diets added with Mycosorb


  • Catootjie Lusje Nalle Feed Technology Study Program, Department of Animal Husbandry, State Polytechnic of Agriculture Kupang
  • Max Arthur Julian Supit Animal Production Study Program, Department of Animal Husbandry, State Polytechnic of Agriculture Kupang
  • Andrijanto Hauferson Angi Animal Health Study Program, Department of Animal Husbandry, State Polytechnic of Agriculture Kupang
  • Beatrix Se-na Bha Feed Technology Study Program, Department of Animal Husbandry, State Polytechnic of Agriculture Kupang
  • Ni Sri Yuliani Feed Technology Study Program, Department of Animal Husbandry, State Polytechnic of Agriculture Kupang



Aflatoxin, Broiler, Mycotoxin binder, Performance, Toxicological effect


The intake of aflatoxin B1 (AFB1) can lead to poor productivity and diseases in birds. Thus, it is important to minimize the toxic effects of AFB1. The objective of this study was to evaluate the carcass yield and haematological profile of broilers fed diets containing AFB1 added with Mycosorb. A total of 240-day old broiler chicks were randomly distributed to 24 pens (10 birds/pen). The experiment was designed using a 4 x 2 factorial arrangement with two different factors, namely aflatoxin level and Mycosorb. The treatments were control diets, control diets added with Mycosorb, diets containing 10.36 ppb AFB1, diets containing 10.36 ppb AFB1 added with Mycosorb, diets containing 26.97 ppb AFB1, diets containing 26.97 ppb AFB1 added with Mycosorb, diets containing 61.06 ppb AFB1, diets containing 61.06 ppb AFB1 added with Mycosorb. There was an interaction (p<0.05 to 0.01) between AFB1 level and Mycosorb on the white blood cells (WBC) and litter/excreta score (LES), but not (p>0.05) in tcarcass traits (CT), lymphoid organ weight (LOW), and haematological profile (HP) of broilers. Level of AFB1 did not affect (p>0.05) all CT, LOW, and HP, but it affected (p<0.001) the LES. Mycosorb did not improve (p>0.05) CT, LOW and HP of broilers.  In conclusions, 1) dietary Mycosorb in afla-treated diets improved litter quality and reduced white blood cell counts of broilers; 2) the AFB1 level up to 61.06 ppb did not impair carcass yield, lymphoid organ weights and other haematological index of broilers.

Author Biography

Catootjie Lusje Nalle, Feed Technology Study Program, Department of Animal Husbandry, State Polytechnic of Agriculture Kupang

Catootjie L. Nalle is a senior lecturer at Polytechnic of Agriculture Kupang. She got her PhD from Massey University, New Zealand.


Abdel-Wahhab, M. A., Nada, S. A., & Khalil, F. A. (2002). Physiological and toxicological responses in rats fed aflatoxin-contaminated diet with or without sorbent materials. Animal Feed Science and Technology, 97(3–4), 209–219. S0377-8401(01)00342-X

AOAC. (2005). Official Methods of Analysis of AOAC International (18th ed.). Assoc. Off. Anal.

Aravind, K., Patil, V., Devegowda, G., Umakantha, B., & Ganpule, S. (2003). Efficacy of esterified glucomannan to counteract mycotoxicosis in naturally contaminated feed on performance and serum biochemical and hematological parameters in broilers. Poultry Science, 82(4), 571–576.

Belay, T., & Teeter, R. G. (1996). Effects of ambient temperature on broiler mineral balance partitioned into urinary and faecal loss. British Poultry Science, 37(2), 423–433. https://doi. org/10.1080/00071669608417873

Benkerroum, N. (2020). Chronic and acute toxicities of aflatoxins: mechanisms of action. International Journal of Environmental Research and Public Health, 17(2), 423. 10.3390/ijerph17020423

Bhatti, S. A., Khan, M. Z., Saleemi, M. K., Saqib, M., Khan, A., & Ul-Hassan, Z. (2017). Protective role of bentonite against aflatoxin B 1 - and ochratoxin A-induced immunotoxicity in broilers. Journal of Immunotoxicology, 14(1), 66–76. 91X.2016.1264503

Bolan, N. S., Szogi, A. A., Chuasavathi, T., Seshadri, B., Rothrock, M. J., & Panneerselvam, P. (2010). Uses and management of poultry litter. World’s Poultry Science Journal, 66(4), 673–698. 33910000656

Cai, P., Zheng, H., She, J., Feng, N., Zou, H., Gu, J., Yuan, Y., Liu, X., Liu, Z., & Bian, J. (2020). Molecular mechanism of aflatoxin-induced hepatocellular carcinoma derived from a bioinformatics analysis. Toxins, 12(3), 203. 10.3390/toxins12030203

Campos-M, M., & Campos-C, R. (2017). Applications of quartering method in soils and foods. International Journal of Engineering Research and Applications, 7(1), 35–39. https://doi. org/10.9790/9622-0701023539

Collett, S. R. (2012). Nutrition and wet litter problems in poultry. Animal Feed Science and Technology, 173(1–2), 65–75. edsci.2011.12.013

de Jong, I. C., van Harn, J., Gunnink, H., Hindle, V. A., & Lourens, A. (2012). Footpad dermatitis in Dutch broiler flocks: Prevalence and factors of influence. Poultry Science, 91(7), 1569–1574. ps.2012-02156

Dunlop, M. W., Moss, A. F., Groves, P. J., Wilkinson, S. J., Stuetz, R. M., & Selle, P. H. (2016). The multidimensional causal factors of ‘wet litter’ in chicken-meat production. Science of The Total Environment, 562, 766–776. https://doi .org/10.1016/j.scitotenv.2016.03.147

Elkatcha, M., Soltan, M., ElShobokshy, S., & Shokry, A. (2017). Protective effect of chemical and biological mycotoxin binder on growth performance, serum biochemistry and carcass traits in broiler chicks fed on aflatoxin contaminated diet. Alexandria Journal of Veterinary Sciences, 55(1), 180–197. https://doi. org/10.5455/ajvs.269041

Etim, N. N. (2014). Haematological parameters and factors affecting their values. Agricultural Science, 2(1), 37–47.

Feng, G. D., He, J., Ao, X., & Chen, D. W. (2017). Effects of maize naturally contaminated with aflatoxin B1 on growth performance, intestinal morphology, and digestive physiology in ducks. Poultry Science, 96(6), 1948–1955. s/pew420

Fouad, A., Ruan, D., El-Senousey, H., Chen, W., Jiang, S., & Zheng, C. (2019). Harmful effects and control strategies of aflatoxin B1 produced by aspergillus flavus and aspergillus parasiticus strains on poultry: review. Toxins, 11(3), 176. 10.3390/toxins11030176

Fountain, J. C., Khera, P., Yang, L., Nayak, S. N., Scully, B. T., Lee, R. D., Chen, Z.-Y., Kemerait, R. C., Varshney, R. K., & Guo, B. (2015). Resistance to Aspergillus flavus in maize and peanut: Molecular biology, breeding, environmental stress, and future perspectives. The Crop Journal, 3(3), 229–237. 2015.02.003

Fowler, J., Li, W., & Bailey, C. (2015). Effects of a calcium bentonite clay in diets containing aflatoxin when measuring liver residues of aflatoxin B1 in starter broiler chicks. Toxins, 7(9), 3455–3464. 3390/toxins7093455

Galarza-Seeber, R., Latorre, J. D., Bielke, L. R., Kuttappan, V. A., Wolfenden, A. D., Hernandez-Velasco, X., Merino-Guzman, R., Vicente, J. L., Donoghue, A., Cross, D., Hargis, B. M., & Tellez, G. (2016). Leaky gut and mycotoxins: aflatoxin B1 does not increase gut permeability in broiler chickens. Frontiers in Veterinary Science, 3. fvets.2016.00010

Grenier, B., & Applegate, T. (2013). Modulation of Intestinal functions following mycotoxin ingestion: meta-analysis of published experiments in animals. Toxins, 5(2), 396–430. https: //

Han, X.-Y., Huang, Q.-C., Li, W.-F., Jiang, J.-F., & Xu, Z.-R. (2008). Changes in growth performance, digestive enzyme activities and nutrient digestibility of cherry valley ducks in response to aflatoxin B1 levels. Livestock Science, 119(1–3), 216–220. 2008.04.006

Hermans, P. G., Fradkin, D., Muchnik, I. B., & Morgan, K. L. (2006). Prevalence of wet litter and the associated risk factors in broiler flocks in the United Kingdom. Veterinary Record, 158(18), 615–622. 1136/vr.158.18.615

Hoeven-Hangoor. (2014). Broiler excreta composition and its effect on wet litter: aspects of nutrition. In Broiler excreta composition and its effect on wet litter: aspects of nutrition. Wangeningen University.

Hoover, N. L., Kanwar, R., Soupir, M. L., & Pederson, C. (2015). Effects of poultry manure application on phosphorus in soil and tile drain water under a corn-soybean rotation. Water, Air, & Soil Pollution, 226(5), 138. 10.1007/s11270-015-2403-9

Hossein, A., & Gürbüz, Y. (2016). Aflatoxins in poultry nutrition. Kahramanmaraş Sütçü İmam Üniversitesi Doğa Bilimleri Dergisi, 18(4), 1. jns.98227

Hussain, Z., Rehman, H.-, Manzoor, S., Tahir, S., & Mukhtar, M. (2016). Determination of liver and muscle aflatoxin B1 residues and select serum chemistry variables during chronic aflatoxicosis in broiler chickens. Veterinary Clinical Pathology, 45(2), 330–334. 12336

Iqbal, S. Z., Asi, M. R., & Ariño, A. (2013). Aflatoxin. In Brenner’s Encyclopedia of Genetics (Ed.), Stanley Maloy and Kelly Hughes (2nd ed., pp. 43–47). Academic Press.

Karimy, M. F., Sutrisno, B., Agus, A., Suryani, A. E., Istiqomah, L., & Damayanti, E. (2017). Aflatoxin effect on erythrocyte profile and histopathology of broilers given different additives. IOP Conference Series: Earth and Environmental Science, 101, 012033. 10.1088/1755-1315/101/1/012033

Khan, M. Z. I., Masum, M., Khan, M. Z. I., Aziz, A. R. Bin, Nasrin, M., Siddique, M. N. H., & Arshad, M. M. Bin. (2014). Histomorphology of the lymphoid tissues of broiler chickens in Kelantan, Malaysia. Sains Malaysiana, 43(8), 1175–1179.

Kheravii, S. K., Swick, R. A., Choct, M., & Wu, S.-B. (2017). Potential of pelleted wheat straw as an alternative bedding material for broilers. Poultry Science, 96(6), 1641–1647. 3382/ps/pew473

Kumar, P., Mahato, D. K., Kamle, M., Mohanta, T. K., & Kang, S. G. (2017). Aflatoxins: a global concern for food safety, human health and their management. Frontiers in Microbiology, 07, 2170. https://d

Kumar, R., & Balachandran, C. (2009). Histopathological changes in broiler chickens fed aflatoxin and cyclopiazonic acid. Veterinarski Arhiv, 79(1), 31–40.

Kumar, V. V. (2018). Aflatoxins: properties, toxicity and detoxification. Nutrition & Food Science International Journal, 6(5). 80/NFSIJ.2018.06.555696

Latimer, G. W. (2005). AOAC International. Official methods of analysis of AOAC International (19th ed.). AOAC International.

Liu, N., Wang, J., Deng, Q., Gu, K., & Wang, J. (2018). Detoxification of aflatoxin B 1 by lactic acid bacteria and hydrated sodium calcium aluminosilicate in broiler chickens. Livestock Science, 208, 28–32.

Marchese, S., Polo, A., Ariano, A., Velotto, S., Costantini, S., & Severino, L. (2018). Aflatoxin B1 and M1: biological properties and their involvement in cancer development. Toxins, 10(6), 214. 10.3390/toxins10060214

Mogadam, N. (2011). Ameliorative effect of glucomannan-containing yeast product (Mycosorb) and sodium bentonite on performance and antibody titers against Newcastle disease in broilers during chronic aflatoxicosis. African Journal Of Biotechnology, 10(75), 17372–17378.

Mohaghegh, A., Chamani, M., Shivazad, M., Sadeghi, A. A., & Afzali, N. (2017). Effect of esterified glucomannan on broilers exposed to natural mycotoxin-contaminated diets. Journal of Applied Animal Research, 45(1), 285–291. https://doi. org/10.1080/09712119.2016.1174122

Moran, C. A., Apajalahti, J., Yiannikouris, A., Ojanperä, S., & Kettunen, H. (2013). Effects of low dietary aflatoxin B1 on broiler liver concentration without and with Mycosorb ® toxin binder. Journal of Applied Animal Nutrition, 2, e4.

Murugesan, G. R., Ledoux, D. R., Naehrer, K., Berthiller, F., Applegate, T. J., Grenier, B., Phillips, T. D., & Schatzmayr, G. (2015). Prevalence and effects of mycotoxins on poultry health and performance, and recent development in mycotoxin counteracting strategies. Poultry Science, 94(6), 1298–1315. https://

Nazarizadeh, H., & Pourreza, J. (2019). Evaluation of three mycotoxin binders to prevent the adverse effects of aflatoxin B 1 in growing broilers. Journal of Applied Animal Research, 47(1), 135–139. 1080/09712119.2019.1584106

Peng, X., Bai, S., Ding, X., Zeng, Q., Zhang, K., & Fang, J. (2015). Pathological changes in the immune organs of broiler chickens fed on corn naturally contaminated with aflatoxins B 1 and B 2. Avian Pathology, 44(3), 192–199.

Prakoso, Y. A., & Kurniasih, K. (2019). Recent update: effects of aflatoxin in broiler chickens. Journal of World’s Poultry Research, 9(2), 68–77.

Raphal, K., Ferdinand, N., Herv, T., Dalex, T., Romo, S., Alexis, T., & Benoit, G. (2014). Effect of biocharcoals on hematological, serum biochemical and histological parameters in broiler chickens fed aflatoxin B1-contaminated diets. Journal of Animal Science Advances, 4(7), 939. https://

Resanovic, R., & Sinovec, Z. (2006). Effects of limited feeding of aflatoxin B1 contaminated feed on the performance of broilers. Mycotoxin Research, 22(3), 183–188. 07/BF02959274

Safameher, A. (2008). Effects of Clinoptilolite on performance, biochemical parameters and hepatic lesions in broiler chickens during aflatoxosis. Journal of Animal and Veterinary Advances, 7(4), 381–388.

Salwa, A. A., & Anwer, W. (2009). Effect of naturally contaminated feed with aflatoxins on performance of laying hens and the carryover of aflatoxin B1 residues in table eggs. Pakistan Journal of Nutrition, 8(2), 181–186. https://

Saminathan, M., Selamat, J., Abbasi Pirouz, A., Abdullah, N., & Zulkifli, I. (2018). Effects of nano-composite adsorbents on the growth performance, serum biochemistry, and organ weights of broilers fed with aflatoxin-contaminated feed. Toxins, 10(9), 345.

Shepherd, E. M., & Fairchild, B. D. (2010). Footpad dermatitis in poultry. Poultry Science, 89(10), 2043–2051. https://

Sineque, A., Macuamule, C., & Dos Anjos, F. (2017). Aflatoxin B1 Contamination in chicken livers and gizzards from industrial and small abattoirs, measured by ELISA Technique in Maputo, Mozambique. International Journal of Environmental Research and Public Health, 14(9), 951. 10.3390/ijerph14090951

Teirlynck, E., Gussem, M. D. E., Dewulf, J., Haesebrouck, F., Ducatelle, R., & Van Immerseel, F. (2011). Morphometric evaluation of “dysbacteriosis†in broilers. Avian Pathology, 40(2), 139–144. 57.2010.543414

Valchev, I., Groseva, N., Kanakov, D., Hristov, T., Lazarov, L., & Binev, R. (2018). Effect of experimentally induced aflatoxicosis on haematological parameters and bone marrow morphology in mulard ducks. Agricultural Science and Technology, 10(3), 208–214. 547/ast.2018.03.040

van Ryssen, J. B. J., Channon, P., & Stielau., W. J. (1977). Minerals and nitrogen in poultry manure. South African Journal of Animal Science, 7, 195–199.

Wade, M. R., Sapcota, D., & Verma, U. (2017). Ameliorating aflatoxicosis in commercial broiler chickens by dietary Mycosorb: Heamato-Biochemical studies. Indian Journal of Animal Research, 3182(00), 1–5.

Wu, H.-C., & Santella, R. (2012). The role of aflatoxins in hepatocellular carcinoma. Hepatitis Monthly, 12(10 HCC). mon.7238

Yang, J., Bai, F., Zhang, K., Lv, X., Bai, S., Zhao, L., Peng, X., Ding, X., Li, Y., & Zhang, J. (2012). Effects of feeding corn naturally contaminated with AFB1 and AFB2 on performance and aflatoxin residues in broilers. Czech Journal of Animal Science, 57(No. 11), 506–515. 1/6383-CJAS

Yunus, A. W., Razzazi-Fazeli, E., & Bohm, J. (2011). Aflatoxin B1 in affecting broiler’s performance, immunity, and gastrointestinal tract: a review of history and contemporary issues. Toxins, 3(6), 566–590. https://doi. org/10.3390/toxins3060566