Volatile fatty acids and methane production in dairy cow ration based on protein-energy synchronization index with a meals protein source

Afduha Nurus Syamsi, Lastriana Waldi


The study was aimed to examine the production of volatile fatty acids (VFA) and methane (CH4) in rations based on protein-energy synchronization index (PES) using different meals protein sources (MPS). The study was conducted experimentally with in vitro techniques. The factorial completely randomized design (CRD) was used to examine six types of treatment rations originating from a combination of 3 PES index and two types of MPS, each repeated four times so that there were a total of 24 experimental units. The results showed that the interaction of the SPE index had no significant effect on the production of partial VFA, but had a significant effect on the acetate: propionate (A: P) ratio and CH4 production. The analysis of honestly significant different (HSD) results showed that the lowest A: P ratio and CH4 were found in R1. The orthogonal test shows that coconut and soybean meal had a quadratic effect on the A: P ratio and CH4. It concluded that the best PES index to optimize VFA and minimize CH4 production was at the index 0.6-0.61 with soybean meal as protein source supplementation.


Volatile fatty acids; Methane; Protein-energy synchronization index; Meal

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Cabrita, A. R. J., Dewhurst, R. J., Abreu, J. M. F., & Fonseca, A. J. M. (2006). Evaluation of the effects of synchronising the availability of N and energy on rumen function and production responses of dairy cows – a review. Animal Research, 55(1), 1–24. https://doi.org/10.1051/animres:2 005045

Chiba, L. I. (2014). Rumen Microbiology and Fermentation. In Animal Nutrition Handbook.

Engelking, L. R. (2015). Overview of Carbohydrate Metabolism. In Textbook of Veterinary Physiological Chemistry (pp. 136–140).

Elsevier. https://doi.org/10.1016/B978-0-12-39 1909-0.50021-9

Goering, H. K., & Soest, P. J. Van. (1970). Forage Fiber Analysis. In Agricultural Handbook (379th ed.). United States Department of Agriculture.

Gulmez, B. H., & Turkmen, I. I. (2007). Effect of starch sources with different degradation rates on ruminal fermentation of lactating dairy cows. Revue de Medecine Veterinaire, 158(2), 92–99.

Hermon, M., Suryahadi, K. G., Wiryawan, & Hardjosoewignjo, S. (2008). Nisbah sinkronisasi suplai N-Protein dan Energi dalam rumen sebagai basis formulasi ransum ternak ruminansia. Media Peternakan, 31(3), 186–194.

Kaswari, T. (2004). Synchronization of Energy and Protein Supply in The Rumen of Diary Crows (3017th ed.). Göttingen.

Mitsumori, M., & Sun, W. (2008). Control of rumen microbial fermentation for mitigating methane emissions from the rumen. Asian-Australasian Journal of Animal Sciences, 21(1), 144–154. https://doi.org/10.5713/ajas. 2008.r01

Ørskov, E. R., & McDonald, I. (1979). The estimating of protein degradability in the rumen from incubation measurement weighted activating to rate of passage. The Journal of Agrculture Science, 92(2), 499–503.

Ørskov, E. R., & Ryle, M. (1990). Energy Nutrition in Ruminants. Elsevier Science Publishers Ltd.

Seo, J. K., Yang, J., Kim, H. J., Upadhaya, S. D., Cho, W. M., & Ha, J. K. (2010). Effects of synchronization of carbohydrate and protein supply on ruminal fermentation, nitrogen metabolism and microbial protein synthesis in holstein steers. Asian-Australasian Journal of Animal Sciences, 23(11), 1455–1461. https:// doi.org/10.5713/ajas.2010.10247

Sinclair, L. A., Garnsworthy, P. C., Newbold, J. R., & Buttery, P. J. (1995). Effects of synchronizing the rate of dietary energy and nitrogen release in diets with a similar carbohydrate composition on rumen fermentation and microbial protein synthesis in sheep. The Journal of Agricultural Science, 124(3), 463–472. https://doi.org/10.1017/S002185 9600073421

Syamsi, A. N., Waldi, L., Widodo, H. S., & Harwanto. (2019). Branched chain volatile fatty acids profile of rumen fluids suplemented by different meal protein sources and protein-energy synchronization index. IOP Conference Series: Earth and Environmental Science, 372(1), 012060. https://doi.org/10.1088/1755-1315/372/1/012060

Syamsi, A.N, Widodo, H. S., Astuti, T. Y., Soediarto, P., & Subagyo, Y. (2020). Potensi Produksi Energi Ransum Berbasis Indeks Sinkronisasi Protein-Energi dengan Suplementasi Leguminosa Berbeda. Prosiding Seminar Nasional Fakultas Agorindustri Tahun 2020: Peningkatan Daya Saing Melalui Perbaikan Mutu Produk Dalam Rangka Pembangunan Pertanian Di Era Industri 4.0.

Syamsi, Afduha Nurus, Astuti, T. Y., & Soediarto, P. (2018). Volatile fatty acids and methane profile of dairy cattle ruminal fluid was gived legumes in ration based on synchronization protein-energy index. Buletin Peternakan, 42(4), 283–289. https:// doi.org/10.21059/buletinpeternak.v42i4.33074

Syamsi, A. N, Suhartati, F. M., & Suryapratama, W. (2019). Pengaruh daun turi (Sesbania grandiflora) dan lamtoro (Leucaena leucocephala) dalam ransum sapi berbasis indeks sinkronisasi protein - energi terhadap sintesis protein mikroba rumen. Pastura, 6(2), 47–52. https://doi.org/ 10.24843/Pastura.2017.v06.i02.p01

Tapio, I., Snelling, T. J., Strozzi, F., & Wallace, R. J. (2017). The ruminal microbiome associated with methane emissions from ruminant livestock. Journal of Animal Science and Biotechnology, 8(1), 1–11. https:// doi.org/10.1186/s40104-017-0141-0

Tilley, J. M. A., & Terry, R. A. (1969). The relationship between the soluble constitutent herbage and their dry matter digestibility. Grass and Forage Science, 24(4), 290–295. https://doi.org/ 10.1111/j.1365-2494.1969.tb01083.x

Waldi, L. (2017). Pengaruh penggunaan bungkil kedelai dan bungkil kelapa dalam ransum berbasis indeks sinkronisasi energi dan protein terhadap sintesis protein mikroba rumen sapi perah. Journal of Livestock Science and Production, 1(1), 1–12. https://doi.org/10.31002/jalspro.v1i1.446

DOI: http://dx.doi.org/10.21776/ub.jiip.2021.031.02.04


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