Expression of estrogen receptors alpha (ERs α) and folliculogenesis profile in ovary of the rats ovarian hypofunction model

Aulia Firmawati, Mitra Artha Kurnia Hutabarat, Herlina Pratiwi, Alibiruni Haryo

Abstract


Ovarian hypofunction is one of the reproductive disorders that occur due to a decrease in ovarian function that causes animals to not experience heat. The purpose of this study was to determine the effect of GnRH antagonist hormone interventions on ovarian hypofunction model rats by looking at the expression of alpha ERs (ERs α) and the profile of folliculogenesis in the ovaries. This study used two groups of female Wistar strain rats, aged 8-10 weeks, bodyweight 150-180 grams and each group contained 10 animals. Intervention gave control group, without cetrorelix acetate intervention (placebo aqua dest sterile), treatment group with the induction of cetrorelix acetate 0.0135 mg/kg BW for 17 days. Observation of α ERs expression in the ovary was analyzed by immunohistochemical methods, and data were analyzed using T-Test analysis (α <0.05). Meanwhile, the folliculogenesis profile was analyzed by hematoxylin-eosin (HE) staining, then analyzed descriptively. The results of this study indicate that the treatment group given the intervention cetrorelix acetate dose 0.0135 mg/kg BW showed significantly different results compared to the control group. In the treatment group, the ERs α expression decreased by 82.7% compared to the control group and in the folliculogenesis profile,  there was a decrease in follicular development from pre-antral follicles to antral follicles. Cetrorelix acetate interventions can have an effect on the inhibition of folliculogenesis and estrogen binding with ERs that cause anoestrous.


Keywords


Ovarian hypofunction; Rat; ERs α; Folliculogenesis

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DOI: http://dx.doi.org/10.21776/ub.jiip.2021.031.01.03

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