Potential of bacteria in sheep feces as biogas formation starter on lignite coal media

Yuli Astuti Hidayati, Marlina ET, Rahmah KN, Pinandito K, Puteri GCK, Harlia E

Abstract


Sheep farms produce waste in the form of feces and urine that are organic materials and the source of bacteria. This study aims to determine the potential of bacteria in sheep feces when used as a starter for biogas formation (the number of anaerobic bacteria and biogas production) on lignite coal media. The method used in this study was experimental in the laboratory using a completely randomized design with four treatments and four replications. The data were analyzed using analysis of variance, and Duncan test was performed to find out the differences between treatments. The research procedure was divided into two stages. The first stage was in vitro method to select the feces sample with the largest biogas production. The second stage was utilizing enriched media (media 98-5) liquid for observing the growth of bacteria originating from selected sheep feces, and then the bacteria consortium was used as starters on lignite coal media. The treatments were based on the concentration of bacteria consortium used, T1=0%, T2=1.5%, T3=3%, and T4=4.5%. The number of anaerobic bacteria was observed in Hungate tubes, and biogas production was observed in serum bottles. The results showed that the highest number of anaerobic bacteria (1012 CFU/ml) and the highest biogas production (105 mL) were achieved at T2=1.5%. Bacteria in sheep feces can be potentially used as a biogas forming agent on lignite coal media, which is proved by the growth of anaerobic bacteria and the biogas production from present study.


Keywords


Sheep feces; Lignite coal; Anaerobic bacteria; Biogas

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References


Akunna, J.C., (2015). Anaerobic treatment of brewery wastes. Brewing Microbiology. Managing Microbes, Ensuring Quality and Valorising Waste.A volume in Woodhead Publishing Series in Food Science, Technology and Nutrition Book

Beauchemin, Yang, W.Z., L.M. Rode. (2003). Effects of Particle Size of Alfalfa-Based Dairy Cow Diets on Chewing Activity, Ruminal Fermentation, and Milk Production, Journal of Dairy Science, February 2003. Volume 86, Issue 2, Pages 630–643

Fayyaz Ali Shah, Qaisar Mahmood, Mohammad Maroof Shah, Arshid Pervez, and Saeed Ahmad Asad. (2014). Microbial Ecology of Anaerobic Digesters: The Key Players of Anaerobiosis, ScientificWorldJournal 2014 : 183752

Hidayati, Y.A., Kurnani Tb.B.A, Marlina, E T, Rahmah, K N and Harlia E., (2018). The activation of microorganism inoculum from rumen of beef cattle, J. Phys.: Conf. Ser.1080 012047

Hidayati, Y. A., Kurnani Tb.B.A, Marlina, E T, Rahmah, K N and Harlia E., (2018). The viability of Anaerobic Bacteria from Beef Cattle Feces in Liquid Media, J. Pow. Tech Adv. Func. Mater. 2018, 1 (1), 24-30, Article in Press. p-ISSN 2621-7821

Lawal A.A., Dzivama A.U., Wasinda M.K. (2016). Effect of inoculum to substrate ratio on biogas production of sheep paunch manure. Res. Agr. Eng., 62: 8-14

Lohani, S. P., dan J. Havukainen. 2018. Anaerobic Digestion: Factors Affecting Anaerobic Digestion Process. In: S. J. Varjani, editor, Waste Bioremediation, Energy, Environment, dan Sustainability. Springer Nature Singapore Pte Ltd, Singapore. p. 343-359

Marlina , E.T. (2009). Bioconversion of Livestock Industry Waste. UNPAD-PRESS Bandung. Indonesia

Marlina, E.T., Kurnani, Tb.B.A., Hidayati, Y.A., Rahmah, K.N. and Harlia, E., (2018). The Potential of Various Livestock Waste as Sources of Methanogenic Bacteria, J. Pow. Tech Adv. Func. Mater. 1 (1), 19-23 p-ISSN 2621-7821

Purnomo A, Suprihatin, M Romli, Udin Hasanudin. 2017. Biogas production from oil palm empty fruit bunches of post mushroom cultivation media. IOP Conf. Se-ries: Earth and Environmental Science 141 (2018) 012024 doi :10.1088/1755-1315/141/1/012024.

Raja. I. A and S. Wazir. 2017. Biogas Production: The Fundamental Processes. Uni-versal Journal of Engineering Science 5(2): 29-37, DOI: 10.13189/ujes.2017.050202

Tianyu Chen, Sandra Rodrigues, Suzanne D.Golding, Victor Rudolph. (2018). Improving coal bioavailability for biogenic methane production via hydrogen peroxide oxidation. International Journal of Coal Geology.Volume 195, Pages 402-414. https://doi.org/10.1016/j.coal.2018.06.011

Vinay K. Sahay. (2018). New Delhi, the fundamental relationship between coal rank and coal type, Journal of the Geological Society of India, vol.75

Zuriana Sidi Ahmad, Mimi Sakinah Abdul Munaim. (2017). Effect Of Fermentation Time, Moisture Content,And Temperature On Sorbitol Production Via Solid State Fermentation Process. Journalof Chemical Engineeringand Industrial Biotechnology V1(2017) 64-71




DOI: http://dx.doi.org/10.21776/ub.jiip.2020.030.03.04

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