Isolasi dan identifikasi Staphylococcus Epidermidis pada susu sapi PFH penderita mastitis subklinis di Wukirsari, Cangkringan, Sleman, DIY

Authors

  • Ervina Ryan Puspasari Fakultas kedokteran Hewan UGM, Yogyakarta
  • Yanuartono - Yanuartono Fakultas kedokteran Hewan UGM, Yogyakarta
  • Sri Hartati Fakultas kedokteran Hewan UGM, Yogyakarta
  • Slamet Rahardjo Fakultas kedokteran Hewan UGM, Yogyakarta
  • Alfarisa Nururrozi Fakultas kedokteran Hewan UGM, Yogyakarta
  • Soedarmanto Indarjulianto Fakultas kedokteran Hewan UGM, Yogyakarta

DOI:

https://doi.org/10.21776/ub.jiip.2018.028.02.04

Keywords:

mastitis subklinis, Californian Mastitis Test, Staphylococcus epidermidis

Abstract

Mastitis is an inflammation of the mammary gland, can be occured clinically or subclinically. One of the causes of mastitis is Staphylococcus epidermidis. This study was aimed to isolate and identify Staphylococcus epidermidis in subclinical mastitis PFH dairy cows in Wukirsari, Cangkringan, Sleman, DIY. Sixteen milk samples were tested with a Californian Mastitis Test (CMT), followed by inoculation in Brain Heart Infusion (BHI), planted on a blood agar plate plate (PAD) and Mac Conkey Agar (MCA) for 24 hours at 370C. The growing colonies were then grown on sugar sugar and nutrient agar (NA) media. Gram staining, catalase test, coagulase test and DNase test as bacteria confirmation.CMT test results showed 15 (93.75%) out of 16 positive milk samples suffered from subclinical mastitis. Results of isolation showed 1 (6.66%)of 16 positive samples infected with Staphylococcus epidermidis.The results of this study indicate that incidence of subclinical mastitis in the study area showed a high rate and is likely to be caused by inadequate management practices and milking procedures. The high incidence of subclinical mastitis is probably caused by other bacteria because only 6.66% is caused by Staphylococcus epidermidis.

Author Biography

Yanuartono - Yanuartono, Fakultas kedokteran Hewan UGM, Yogyakarta

departemen Ilmu Penyakit Dalam, FKH-UGM

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Published

2018-08-17

How to Cite

Puspasari, E. R., Yanuartono, Y. .-., Hartati, S., Rahardjo, S., Nururrozi, A., & Indarjulianto, S. (2018). Isolasi dan identifikasi Staphylococcus Epidermidis pada susu sapi PFH penderita mastitis subklinis di Wukirsari, Cangkringan, Sleman, DIY. Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science), 28(2), 121–128. https://doi.org/10.21776/ub.jiip.2018.028.02.04

Issue

Vol. 28 No. 2 (2018): Agustus

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