The effectiveness of cassava leaf meal (Manihot esculenta Crantz) in feed with enzymes supplementation on Broiler digestive organs




Broiler, Cassava leaf meal, Digestive organ, Enzyme, Local feedstuffs


This research aimed to evaluate the effect of cassava leaf meal with enzyme supplementation in feed on the digestive organs of the broiler. The research used a completely randomized factorial design on 48 Cobb-strain broilers distributed into two observation factors and four replications (consisting of 2 samples). The observation factor is factor 1 is the addition of cassava leaf meal (0%; 1.5%; and 3.0%), and the second factor is the addition of non-starch polysaccharides and protease enzymes with each dose of 250 g/ton feed (with and without enzymes). The variables analyzed were the relative weight of the digestive organs, the relative weight of the parts of the small intestine, and the relative length of the parts of the small intestine. The data obtained were analyzed by analysis of variance (ANOVA), and if the results indicated significantly different, then the posthoc test was conducted. The results showed that the addition of cassava leaf meal could increase the relative weight of the gizzard significantly (p<0.05). The addition of enzymes can significantly decrease the relative weight of the gizzard, small intestine, pancreas, ileum, and the relative length of the duodenum and ileum (p<0.05). The use of cassava leaf meal in the feed up to 3.0% does not interfere with digestive organs. There was no interaction between cassava leaf meal and enzymes on size response of digestibility broiler organ. The addition of enzymes can offset the detrimental effects caused by the use of cassava leaf meals in feed.

Author Biography

Muhammad Ridla, Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan, IPB University Kmapus Dramaga Bogor 16680

Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan, IPB University Kampus Dramaga Bogor 16680


AOAC. (2005). Official Methods of Analysis. Association of Official Analytical Chemists Inc.

Badan Pusat Statistik. (2019). Impor Kedelai menurut Negara Asal Utama 2010-2018.

Badan Standarisasi Nasional. (1992). Cara Uji Makanan dan Minuman.

Badan Standarisasi Nasional. (2015). Pakan Ayam Ras Pedaging (Broiler).

Cho, H. M., Hong, J. S., Kim, Y. Bin, Nawarathne, S. R., Choi, I., Yi, Y.-J., Wu, D., Lee, H., Han, S. E., Nam, K. T., Seoung, E. Il, & Heo, J. M. (2020). Responses in growth performance and nutrient digestibility to a multi-protease supplementation in amino acid-deficient broiler diets. Journal of Animal Science and Technology, 62(6), 840–853. 87/jast.2020.62.6.840

da Silva Santos, B. R., Requião Silva, E. F., Minho, L. A. C., Brandão, G. C., Pinto dos Santos, A. M., Carvalho dos Santos, W. P., Lopes Silva, M. V., & Lopes dos Santos, W. N. (2020). Evaluation of the nutritional composition in effect of processing cassava leaves (Manihot esculenta) using multivariate analysis techniques. Microchemical Journal, 152, 104271. j.microc.2019.104271

Dianti, D. (2012). Pengaruh pemberian ransum dengan beberapa level serat kasar pada periode awal terhadap bobot organ fisiologis ayam broiler pasca pemberian ransum normal. Jurnal Teknologi Pertanian, 1(2), 28–37.

Diarra, S. S., & Anand, S. (2020). Impact of commercial feed dilution with copra meal or cassava leaf meal and enzyme supplementation on broiler performance. Poultry Science, 99(11), 5867–5873. .psj.2020.08.028

Ding, X. M., Li, D. D., Li, Z. R., Wang, J. P., Zeng, Q. F., Bai, S. P., Su, Z. W., & Zhang, K. Y. (2016). Effects of dietary crude protein levels and exogenous protease on performance, nutrient digestibility, trypsin activity and intestinal morphology in broilers. Livestock Science, 193, 26–31. https: //

Du, T. H., & Preston, T. R. (2005). The effects of simple processing methods of cassava leaves on HCN content and intake by growing pigs. Livestock Research for Rural Development, 17(9).

Erdaw, M. M., Perez-Maldonado, R. A., Bhuiyan, M., & Iji, P. A. (2017). Partial replacement of commercial soybean meal with raw, full-fat soybean meal supplemented with varying levels of protease in diets of broiler chickens. South African Journal of Animal Science, 47(1), 61.

Erdaw, M. M., Perez-Maldonado, R. A., & Iji, P. A. (2019). Protease and phytase supplementation of broiler diets in which soybean meal is partially or completely replaced by raw full-fat soybean. South African Journal of Animal Science, 49(3), 455.

Essien, E. B., Enyenihi, G. E., & Inyang, U. A. (2018). Carcass yield and internal organs of broilers fed graded levels of full fat palm fruit meal diets with and without enzyme. Nigerian Journal Animal Science, 20(4), 594–600.

Fasuyi, A. O. (2004). Nutrient composition and processing effects on cassava leaf (Manihot esculenta, Crantz) antinutrients. Pakistan Journal of Nutrition, 4(1), 37–42. https://doi. org/10.3923/pjn.2005.37.42

Ghazi, S., Rooke, J. A., Galbraith, H., & Bedford, M. R. (2002). The potential for the improvement of the nutritive value of soya-bean meal by different proteases in broiler chicks and broiler cockerels. British Poultry Science, 43(1), 70–77. 80/00071660120109935

Has, H., Napirah, A., & Indi, A. (2015). Efek peningkatan serat kasar dengan penggunaan daun murbei dalam ransum broiler terhadap persentase bobot saluran pencernaan. Jurnal Ilmu Dan Teknologi Peternakan Tropis, 1(1), 63. .v1i1.362

Hermanto, H., & Fitriani, F. (2018). Pengaruh lama proses fermentasi terhadap kadar asam sianida (HCN) dan kadar protein pada kulit dan daun singkong. Jurnal Riset Teknologi Industri, 12(2), 169–180. https://doi. org/10.26578/jrti.v12i2.4239

Hetland, H., Svihus, B., & Choct, M. (2005). Role of insoluble fiber on gizzard activity in layers. Journal of Applied Poultry Research, 14(1), 38–46.

Hidayat, C. (2009). Peluang penggunaan kulit singkong sebagai pakan unggas. Seminar Nasional Teknologi Peternakan Dan Veteriner, 655 – 665.

Kementrian Pertanian. (2018). Produksi Ubi Kayu Menurut Provinsi, 2014 – 2018.

Madalla, N., Agbo, N. W., & Jauncey, K. (2016). Evaluaton of ground –sundried cassava leaf meal as protein source for nile tilapia Oreochrois nilotics (L) juvenile’s diet. Tanzania Journal of Agricultural Sci, 15(1), 1–12.

Makhdum, Z., Habib-ur-Rehman, Larik, J. M., Bux, P., & Hameed, A. (2013). Crude enzymes supplementation in fibrous diet improves performance of commercial broilers. Journal of Applied Animal Research, 41(2), 218–222. 9.2012.742439

Modesto Junior, E. N., Chisté, R. C., & Pena, R. da S. (2019). Oven drying and hot water cooking processes decrease HCN contents of cassava leaves. Food Research International, 119, 517–523. /j.foodres.2019.01.029

Morgan, N. K., & Choct, M. (2016). Cassava: Nutrient composition and nutritive value in poultry diets. Animal Nutrition, 2(4), 253–261. https://doi. org/10.1016/j.aninu.2016.08.010

Oluwafemi, R. A., & Omaku, G. (2017). Nutritional evaluation of cassava leaf meal based diets on broiler starter chicks performance. International Journal of Food Science and Nutrition, 2(2), 42–45.

Salu, B., & Paembonan, B. H. (2010). Pengaruh pemberian tepung daun ubi kayu (Manihot utilissima) terhadap performans ayam broiler. AgroSaint UKI Toraja, 1(2), 36–46.

Toghyani, M., Wu, S. B., Pérez-Maldonado, R. A., Iji, P. A., & Swick, R. A. (2017). Performance, nutrient utilization, and energy partitioning in broiler chickens offered high canola meal diets supplemented with multicomponent carbohydrase and mono-component protease. Poultry Science, 96(11), 3960–3972. https://

Wang, X., Farnell, Y. Z., Peebles, E. D., Kiess, A. S., Wamsley, K. G. S., & Zhai, W. (2016). Effects of prebiotics, probiotics, and their combination on growth performance, small intestine morphology, and resident Lactobacillus of male broilers. Poultry Science, 95(6), 1332–1340. https://

Widjaya, N. (2017). Pengaruh tingkat penambahan tepung daun singkong dalam ransum komersial terhadap bobot ampela, jantung dan hati broiler strain CP 707. Sains Peternakan, 10(1), 7. sainspet.v10i1.4806

Wobeto, C., Corrêa, A. D., Abreu, C. M. P. de, Santos, C. D. dos, & Abreu, J. R. de. (2006). Nutrients in the cassava (Manihot esculenta Crantz) leaf meal at three ages of the plant. Ciência e Tecnologia de Alimentos, 26(4), 865–869.

Yakubu, B., Mbahi, T. F., Bako, M. I., & Antyev, M. (2018). Effects of dietary levels of enzyme (Maxigrain®) supplemented yam peel meal on performance and carcass characteristics of weaner rabbits. Global Journal of Agricultural Sciences, 16(1), 11. 4314/gjass.v16i1.2

Yang, H. M., Wang, W., Wang, Z. Y., Wang, J., Cao, Y. J., & Chen, Y. H. (2013). Comparative study of intestine length, weight and digestibility on different body weight chickens. African Journal of Biotechnology, 12(32), 5097–5100. 10.5897/AJB11.4014

Yuan, J., Yao, J., Yang, F., Yang, X., Wan, X., Han, J., Wang, Y., Chen, X., Liu, Y., Zhou, Z., Zhou, N., & Feng, X. (2008). Effects of supplementing different levels of a commercial enzyme complex on performance, nutrient availability, enzyme activity and gut morphology of broilers. Asian-Australasian Journal of Animal Sciences, 21(5), 692–700. https://doi. org/10.5713/ajas.2008.70375

Yuan, L., Wang, M., Zhang, X., & Wang, Z. (2017). Effects of protease and non-starch polysaccharide enzyme on performance, digestive function, activity and gene expression of endogenous enzyme of broilers. PLOS ONE, 12(3), e0173941. /10.1371/journal.pone.0173941